diff --git a/tests/bsim/bluetooth/host/misc/hfc_multilink/dut/Kconfig.sysbuild b/tests/bsim/bluetooth/host/misc/hfc_multilink/dut/Kconfig.sysbuild
new file mode 100644
index 00000000000..79d8f991960
--- /dev/null
+++ b/tests/bsim/bluetooth/host/misc/hfc_multilink/dut/Kconfig.sysbuild
@@ -0,0 +1,14 @@
+# Copyright 2025 Nordic Semiconductor ASA
+# SPDX-License-Identifier: Apache-2.0
+
+source "share/sysbuild/Kconfig"
+
+config NET_CORE_BOARD
+	string
+	default "nrf5340bsim/nrf5340/cpunet" if $(BOARD_TARGET_STRING) = "NRF5340BSIM_NRF5340_CPUAPP"
+
+config NATIVE_SIMULATOR_PRIMARY_MCU_INDEX
+	int
+	# Let's pass the test arguments to the application MCU test
+	# otherwise by default they would have gone to the net core.
+	default 0 if $(BOARD_TARGET_STRING) = "NRF5340BSIM_NRF5340_CPUAPP"
diff --git a/tests/bsim/bluetooth/host/misc/hfc_multilink/dut/nrf5340_cpunet-bt_ll_sw_split.conf b/tests/bsim/bluetooth/host/misc/hfc_multilink/dut/nrf5340_cpunet-bt_ll_sw_split.conf
new file mode 100644
index 00000000000..5f45c3c8856
--- /dev/null
+++ b/tests/bsim/bluetooth/host/misc/hfc_multilink/dut/nrf5340_cpunet-bt_ll_sw_split.conf
@@ -0,0 +1,24 @@
+CONFIG_IPC_SERVICE=y
+CONFIG_MBOX=y
+
+CONFIG_ISR_STACK_SIZE=1024
+CONFIG_IDLE_STACK_SIZE=256
+CONFIG_MAIN_STACK_SIZE=512
+CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
+CONFIG_IPC_SERVICE_BACKEND_RPMSG_WQ_STACK_SIZE=512
+CONFIG_HEAP_MEM_POOL_SIZE=8192
+CONFIG_CBPRINTF_REDUCED_INTEGRAL=y
+
+CONFIG_ISR_TABLES_LOCAL_DECLARATION=y
+CONFIG_LTO=y
+
+CONFIG_BT=y
+CONFIG_BT_HCI_RAW=y
+CONFIG_BT_MAX_CONN=3
+
+# Host features
+CONFIG_BT_EXT_ADV=y
+
+# Controller
+CONFIG_BT_LL_SW_SPLIT=y
+CONFIG_BT_CTLR_ASSERT_HANDLER=y
diff --git a/tests/bsim/bluetooth/host/misc/hfc_multilink/dut/sysbuild.cmake b/tests/bsim/bluetooth/host/misc/hfc_multilink/dut/sysbuild.cmake
new file mode 100644
index 00000000000..38b24316936
--- /dev/null
+++ b/tests/bsim/bluetooth/host/misc/hfc_multilink/dut/sysbuild.cmake
@@ -0,0 +1,20 @@
+# Copyright (c) 2025 Nordic Semiconductor ASA
+# SPDX-License-Identifier: Apache-2.0
+
+if(NOT("${SB_CONFIG_NET_CORE_BOARD}" STREQUAL ""))
+	set(NET_APP hci_ipc)
+	set(NET_APP_SRC_DIR ${ZEPHYR_BASE}/samples/bluetooth/${NET_APP})
+
+	ExternalZephyrProject_Add(
+		APPLICATION ${NET_APP}
+		SOURCE_DIR  ${NET_APP_SRC_DIR}
+		BOARD       ${SB_CONFIG_NET_CORE_BOARD}
+	)
+
+	set(${NET_APP}_CONF_FILE ${APP_DIR}/nrf5340_cpunet-bt_ll_sw_split.conf CACHE INTERNAL "")
+
+	native_simulator_set_primary_mcu_index(${DEFAULT_IMAGE} ${NET_APP})
+	native_simulator_set_child_images(${DEFAULT_IMAGE} ${NET_APP})
+endif()
+
+native_simulator_set_final_executable(${DEFAULT_IMAGE})
diff --git a/tests/bsim/bluetooth/host/misc/hfc_multilink/dut/testcase.yaml b/tests/bsim/bluetooth/host/misc/hfc_multilink/dut/testcase.yaml
index 2cb24db1258..2cefbebb87b 100644
--- a/tests/bsim/bluetooth/host/misc/hfc_multilink/dut/testcase.yaml
+++ b/tests/bsim/bluetooth/host/misc/hfc_multilink/dut/testcase.yaml
@@ -1,10 +1,12 @@
 tests:
   bluetooth.host.misc.hfc_multilink.dut:
     build_only: true
+    sysbuild: true
     tags:
       - bluetooth
     platform_allow:
       - nrf52_bsim/native
+      - nrf5340bsim/nrf5340/cpuapp
     harness: bsim
     harness_config:
       bsim_exe_name: tests_bsim_bluetooth_host_misc_hfc_multilink_dut_prj_conf
diff --git a/tests/bsim/bluetooth/host/misc/hfc_multilink/tester/Kconfig.sysbuild b/tests/bsim/bluetooth/host/misc/hfc_multilink/tester/Kconfig.sysbuild
new file mode 100644
index 00000000000..79d8f991960
--- /dev/null
+++ b/tests/bsim/bluetooth/host/misc/hfc_multilink/tester/Kconfig.sysbuild
@@ -0,0 +1,14 @@
+# Copyright 2025 Nordic Semiconductor ASA
+# SPDX-License-Identifier: Apache-2.0
+
+source "share/sysbuild/Kconfig"
+
+config NET_CORE_BOARD
+	string
+	default "nrf5340bsim/nrf5340/cpunet" if $(BOARD_TARGET_STRING) = "NRF5340BSIM_NRF5340_CPUAPP"
+
+config NATIVE_SIMULATOR_PRIMARY_MCU_INDEX
+	int
+	# Let's pass the test arguments to the application MCU test
+	# otherwise by default they would have gone to the net core.
+	default 0 if $(BOARD_TARGET_STRING) = "NRF5340BSIM_NRF5340_CPUAPP"
diff --git a/tests/bsim/bluetooth/host/misc/hfc_multilink/tester/nrf5340_cpunet-bt_ll_sw_split.conf b/tests/bsim/bluetooth/host/misc/hfc_multilink/tester/nrf5340_cpunet-bt_ll_sw_split.conf
new file mode 100644
index 00000000000..6f0462852d5
--- /dev/null
+++ b/tests/bsim/bluetooth/host/misc/hfc_multilink/tester/nrf5340_cpunet-bt_ll_sw_split.conf
@@ -0,0 +1,28 @@
+CONFIG_IPC_SERVICE=y
+CONFIG_MBOX=y
+
+CONFIG_ISR_STACK_SIZE=1024
+CONFIG_IDLE_STACK_SIZE=256
+CONFIG_MAIN_STACK_SIZE=512
+CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
+CONFIG_IPC_SERVICE_BACKEND_RPMSG_WQ_STACK_SIZE=512
+CONFIG_HEAP_MEM_POOL_SIZE=8192
+CONFIG_CBPRINTF_REDUCED_INTEGRAL=y
+
+CONFIG_ISR_TABLES_LOCAL_DECLARATION=y
+CONFIG_LTO=y
+
+CONFIG_BT=y
+CONFIG_BT_HCI_RAW=y
+CONFIG_BT_MAX_CONN=1
+
+# Host features
+CONFIG_BT_EXT_ADV=y
+
+# Controller
+CONFIG_BT_LL_SW_SPLIT=y
+CONFIG_BT_CTLR_ASSERT_HANDLER=y
+
+CONFIG_BT_BUF_ACL_RX_SIZE=255
+CONFIG_BT_BUF_CMD_TX_SIZE=255
+CONFIG_BT_BUF_EVT_DISCARDABLE_SIZE=255
diff --git a/tests/bsim/bluetooth/host/misc/hfc_multilink/tester/sysbuild.cmake b/tests/bsim/bluetooth/host/misc/hfc_multilink/tester/sysbuild.cmake
new file mode 100644
index 00000000000..38b24316936
--- /dev/null
+++ b/tests/bsim/bluetooth/host/misc/hfc_multilink/tester/sysbuild.cmake
@@ -0,0 +1,20 @@
+# Copyright (c) 2025 Nordic Semiconductor ASA
+# SPDX-License-Identifier: Apache-2.0
+
+if(NOT("${SB_CONFIG_NET_CORE_BOARD}" STREQUAL ""))
+	set(NET_APP hci_ipc)
+	set(NET_APP_SRC_DIR ${ZEPHYR_BASE}/samples/bluetooth/${NET_APP})
+
+	ExternalZephyrProject_Add(
+		APPLICATION ${NET_APP}
+		SOURCE_DIR  ${NET_APP_SRC_DIR}
+		BOARD       ${SB_CONFIG_NET_CORE_BOARD}
+	)
+
+	set(${NET_APP}_CONF_FILE ${APP_DIR}/nrf5340_cpunet-bt_ll_sw_split.conf CACHE INTERNAL "")
+
+	native_simulator_set_primary_mcu_index(${DEFAULT_IMAGE} ${NET_APP})
+	native_simulator_set_child_images(${DEFAULT_IMAGE} ${NET_APP})
+endif()
+
+native_simulator_set_final_executable(${DEFAULT_IMAGE})
diff --git a/tests/bsim/bluetooth/host/misc/hfc_multilink/tester/testcase.yaml b/tests/bsim/bluetooth/host/misc/hfc_multilink/tester/testcase.yaml
index b9dd7b87913..a62076ecfdc 100644
--- a/tests/bsim/bluetooth/host/misc/hfc_multilink/tester/testcase.yaml
+++ b/tests/bsim/bluetooth/host/misc/hfc_multilink/tester/testcase.yaml
@@ -1,10 +1,12 @@
 tests:
   bluetooth.host.misc.hfc_multilink.tester:
     build_only: true
+    sysbuild: true
     tags:
       - bluetooth
     platform_allow:
       - nrf52_bsim/native
+      - nrf5340bsim/nrf5340/cpuapp
     harness: bsim
     harness_config:
       bsim_exe_name: tests_bsim_bluetooth_host_misc_hfc_multilink_tester_prj_conf
diff --git a/tests/bsim/bluetooth/tests.nrf5340bsim_nrf5340_cpuapp.txt b/tests/bsim/bluetooth/tests.nrf5340bsim_nrf5340_cpuapp.txt
index b3a15c14d81..6b2ac3ff03a 100644
--- a/tests/bsim/bluetooth/tests.nrf5340bsim_nrf5340_cpuapp.txt
+++ b/tests/bsim/bluetooth/tests.nrf5340bsim_nrf5340_cpuapp.txt
@@ -11,4 +11,5 @@ tests/bsim/bluetooth/audio_samples/
 tests/bsim/bluetooth/audio/
 tests/bsim/bluetooth/host/gatt/notify_stress/
 tests/bsim/bluetooth/host/misc/hfc/
+tests/bsim/bluetooth/host/misc/hfc_multilink/
 tests/bsim/bluetooth/tester/