diff --git a/tests/bsim/bluetooth/host/gatt/ccc_store/overlay-no_long_wq.conf b/tests/bsim/bluetooth/host/gatt/ccc_store/overlay-no_long_wq.conf
new file mode 100644
index 00000000000..baebf9ca980
--- /dev/null
+++ b/tests/bsim/bluetooth/host/gatt/ccc_store/overlay-no_long_wq.conf
@@ -0,0 +1,2 @@
+# Disable the dedicated workqueue for long-running tasks.
+CONFIG_BT_LONG_WQ=n
diff --git a/tests/bsim/bluetooth/host/gatt/ccc_store/test_scripts/ccc_store_no_long_wq.sh b/tests/bsim/bluetooth/host/gatt/ccc_store/test_scripts/ccc_store_no_long_wq.sh
new file mode 100755
index 00000000000..cad38e56e52
--- /dev/null
+++ b/tests/bsim/bluetooth/host/gatt/ccc_store/test_scripts/ccc_store_no_long_wq.sh
@@ -0,0 +1,41 @@
+#!/bin/env bash
+# Copyright 2025 Nordic Semiconductor ASA
+# SPDX-License-Identifier: Apache-2.0
+
+source ${ZEPHYR_BASE}/tests/bsim/sh_common.source
+
+test_exe="bs_${BOARD_TS}_$(guess_test_long_name)_overlay-no_long_wq_conf"
+simulation_id="ccc_store_no_long_wq"
+verbosity_level=2
+EXECUTE_TIMEOUT=60
+
+cd ${BSIM_OUT_PATH}/bin
+
+if [ "${1}" != 'debug0' ]; then
+  Execute "./${test_exe}" \
+    -v=${verbosity_level} -s=${simulation_id} -d=0 -testid=central \
+    -flash="${simulation_id}_client.log.bin" -flash_rm -RealEncryption=1 -argstest 10
+fi
+
+if [ "${1}" != 'debug1' ]; then
+  Execute "./${test_exe}" \
+    -v=${verbosity_level} -s=${simulation_id} -d=1 -testid=peripheral \
+    -flash="${simulation_id}_server.log.bin" -flash_rm -RealEncryption=1 -argstest 10
+fi
+
+Execute ./bs_2G4_phy_v1 -v=${verbosity_level} -s=${simulation_id} \
+  -D=2 -sim_length=60e6
+
+if [ "${1}" == 'debug0' ]; then
+  gdb --args "./${test_exe}" \
+    -v=${verbosity_level} -s=${simulation_id} -d=0 -testid=central \
+    -flash="${simulation_id}_client.log.bin" -flash_rm -RealEncryption=1 -argstest 10
+fi
+
+if [ "${1}" == 'debug1' ]; then
+  gdb --args "./${test_exe}" \
+    -v=${verbosity_level} -s=${simulation_id} -d=1 -testid=peripheral \
+    -flash="${simulation_id}_server.log.bin" -flash_rm -RealEncryption=1 -argstest 10
+fi
+
+wait_for_background_jobs
diff --git a/tests/bsim/bluetooth/host/gatt/ccc_store/test_scripts/ccc_no_store_on_write.sh b/tests/bsim/bluetooth/host/gatt/ccc_store/test_scripts/ccc_store_no_store_on_write.sh
similarity index 96%
rename from tests/bsim/bluetooth/host/gatt/ccc_store/test_scripts/ccc_no_store_on_write.sh
rename to tests/bsim/bluetooth/host/gatt/ccc_store/test_scripts/ccc_store_no_store_on_write.sh
index 97b6631b1a4..b732d1c598f 100755
--- a/tests/bsim/bluetooth/host/gatt/ccc_store/test_scripts/ccc_no_store_on_write.sh
+++ b/tests/bsim/bluetooth/host/gatt/ccc_store/test_scripts/ccc_store_no_store_on_write.sh
@@ -5,7 +5,7 @@
 source ${ZEPHYR_BASE}/tests/bsim/sh_common.source
 
 test_exe="bs_${BOARD_TS}_$(guess_test_long_name)_overlay-no_store_on_write_conf"
-simulation_id="ccc_no_store_on_write"
+simulation_id="ccc_store_no_store_on_write"
 verbosity_level=2
 EXECUTE_TIMEOUT=60
 
diff --git a/tests/bsim/bluetooth/host/gatt/ccc_store/testcase.yaml b/tests/bsim/bluetooth/host/gatt/ccc_store/testcase.yaml
index 12ab2fc7036..a2ee367de1a 100644
--- a/tests/bsim/bluetooth/host/gatt/ccc_store/testcase.yaml
+++ b/tests/bsim/bluetooth/host/gatt/ccc_store/testcase.yaml
@@ -10,8 +10,13 @@ tests:
   bluetooth.host.gatt.ccc_store:
     harness_config:
       bsim_exe_name: tests_bsim_bluetooth_host_gatt_ccc_store_prj_conf
-  bluetooth.host.gatt.ccc_store_2:
+  bluetooth.host.gatt.ccc_store_no_store_on_write:
     harness_config:
       bsim_exe_name: tests_bsim_bluetooth_host_gatt_ccc_store_overlay-no_store_on_write_conf
     extra_args:
       EXTRA_CONF_FILE=overlay-no_store_on_write.conf
+  bluetooth.host.gatt.ccc_store_no_long_wq:
+    harness_config:
+      bsim_exe_name: tests_bsim_bluetooth_host_gatt_ccc_store_overlay-no_long_wq_conf
+    extra_args:
+      EXTRA_CONF_FILE=overlay-no_long_wq.conf