diff --git a/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0-pinctrl.dtsi b/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0-pinctrl.dtsi
index 08c5e112da6..25465279e35 100644
--- a/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0-pinctrl.dtsi
+++ b/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0-pinctrl.dtsi
@@ -24,6 +24,22 @@
 	i2c0_sda: i2c0_sda_default {
 		pinmux = <K3_PINMUX(0x264, PIN_INPUT_PULLUP, MUX_MODE_0)>;
 	};
+
+	spi0_cs0: spi0_cs0_default {
+		pinmux = <K3_PINMUX(0x208, PIN_OUTPUT, MUX_MODE_0)>;
+	};
+
+	spi0_clk: spi0_clk_default {
+		pinmux = <K3_PINMUX(0x210, PIN_OUTPUT, MUX_MODE_0)>;
+	};
+
+	spi0_d0: spi0_d0_default {
+		pinmux = <K3_PINMUX(0x214, PIN_INPUT, MUX_MODE_0)>;
+	};
+
+	spi0_d1: spi0_d1_default {
+		pinmux = <K3_PINMUX(0x218, PIN_OUTPUT, MUX_MODE_0)>;
+	};
 };
 
 &mcu_pinctrl {
diff --git a/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0.dts b/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0.dts
index da484a8ea2f..862c7ff18e7 100644
--- a/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0.dts
+++ b/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0.dts
@@ -89,6 +89,12 @@
 	power-domains = <&i2c0_pd>;
 };
 
+&mcspi0 {
+	pinctrl-0 = <&spi0_clk &spi0_cs0 &spi0_d0 &spi0_d1>;
+	pinctrl-names = "default";
+	status = "okay";
+};
+
 &adc0 {
 	ti,vrefp = <1800>;
 	ti,fifo = <0>;
diff --git a/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0.yaml b/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0.yaml
index 11fe527a8e8..4fbc6ad891c 100644
--- a/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0.yaml
+++ b/boards/ti/am243x_evm/am243x_evm_am2434_r5f0_0.yaml
@@ -10,4 +10,6 @@ ram: 256
 toolchain:
   - zephyr
   - gnuarmemb
+supported:
+  - spi
 vendor: ti
diff --git a/dts/arm/ti/am64x_main.dtsi b/dts/arm/ti/am64x_main.dtsi
index 3da10fe1f8a..232366dca4b 100644
--- a/dts/arm/ti/am64x_main.dtsi
+++ b/dts/arm/ti/am64x_main.dtsi
@@ -215,6 +215,16 @@
 		status = "disabled";
 	};
 
+	mcspi0: spi@20100000 {
+		compatible = "ti,omap-mcspi";
+		reg = <0x20100000 0x400>;
+		clock-frequency = <DT_FREQ_M(50)>;
+		ti,spi-num-cs = <4>;
+		#address-cells = <1>;
+		#size-cells = <0>;
+		status = "disabled";
+	};
+
 	adc0: adc@28001000 {
 		compatible = "ti,am335x-adc";
 		reg = <0x28001000 DT_SIZE_K(1)>;
diff --git a/tests/drivers/spi/spi_loopback/boards/am243x_evm_am2434_r5f0_0.conf b/tests/drivers/spi/spi_loopback/boards/am243x_evm_am2434_r5f0_0.conf
new file mode 100644
index 00000000000..4f7262e78d5
--- /dev/null
+++ b/tests/drivers/spi/spi_loopback/boards/am243x_evm_am2434_r5f0_0.conf
@@ -0,0 +1,6 @@
+# Copyright (c) 2025 Texas Instruments
+#
+# SPDX-License-Identifier: Apache-2.0
+
+CONFIG_SPI_LOOPBACK_MODE_LOOP=y
+CONFIG_SPI_IDEAL_TRANSFER_DURATION_SCALING=25
diff --git a/tests/drivers/spi/spi_loopback/boards/am243x_evm_am2434_r5f0_0.overlay b/tests/drivers/spi/spi_loopback/boards/am243x_evm_am2434_r5f0_0.overlay
new file mode 100644
index 00000000000..96dbdca4c9f
--- /dev/null
+++ b/tests/drivers/spi/spi_loopback/boards/am243x_evm_am2434_r5f0_0.overlay
@@ -0,0 +1,21 @@
+/*
+ * Copyright (c) 2025 Texas Instruments
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+#include <freq.h>
+
+&mcspi0 {
+	slow@0 {
+		compatible = "test-spi-loopback-slow";
+		reg = <0>;
+		spi-max-frequency = <DT_FREQ_K(500)>;
+	};
+
+	fast@0 {
+		compatible = "test-spi-loopback-fast";
+		reg = <0>;
+		spi-max-frequency = <DT_FREQ_M(50)>;
+	};
+};