diff --git a/scripts/pylib/twister/twisterlib/hardwaremap.py b/scripts/pylib/twister/twisterlib/hardwaremap.py
index a0ea7c59a8e..5e90b7c84c6 100644
--- a/scripts/pylib/twister/twisterlib/hardwaremap.py
+++ b/scripts/pylib/twister/twisterlib/hardwaremap.py
@@ -13,6 +13,7 @@ import yaml
 import scl
 import logging
 from pathlib import Path
+from natsort import natsorted
 
 from twisterlib.environment import ZEPHYR_BASE
 
@@ -321,7 +322,7 @@ class HardwareMap:
 
     def save(self, hwm_file):
         # use existing map
-        self.detected.sort(key=lambda x: x.serial or '')
+        self.detected = natsorted(self.detected, key=lambda x: x.serial or '')
         if os.path.exists(hwm_file):
             with open(hwm_file, 'r') as yaml_file:
                 hwm = yaml.load(yaml_file, Loader=SafeLoader)
diff --git a/scripts/requirements-run-test.txt b/scripts/requirements-run-test.txt
index 3bb0b21c44e..83692128110 100644
--- a/scripts/requirements-run-test.txt
+++ b/scripts/requirements-run-test.txt
@@ -7,6 +7,7 @@ pyocd>=0.35.0
 
 # used by twister for board/hardware map
 tabulate
+natsort
 
 # used by mcuboot
 cbor>=1.0.0