diff --git a/subsys/bluetooth/controller/ll_sw/nordic/lll/lll_peripheral_iso.c b/subsys/bluetooth/controller/ll_sw/nordic/lll/lll_peripheral_iso.c
index 22f8f52fda0..bb17e2efbc1 100644
--- a/subsys/bluetooth/controller/ll_sw/nordic/lll/lll_peripheral_iso.c
+++ b/subsys/bluetooth/controller/ll_sw/nordic/lll/lll_peripheral_iso.c
@@ -1012,6 +1012,8 @@ static void next_cis_prepare(void *param)
 		return;
 	}
 
+	payload_count_rx_flush_or_txrx_inc(cis_lll);
+
 	cis_handle_curr = cis_handle;
 
 	radio_isr_set(isr_prepare_subevent_next_cis, next_cis_lll);