diff --git a/drivers/i2c/i2c_nrfx_twim.c b/drivers/i2c/i2c_nrfx_twim.c
index d5b619bfc5a..b6c37f455dc 100644
--- a/drivers/i2c/i2c_nrfx_twim.c
+++ b/drivers/i2c/i2c_nrfx_twim.c
@@ -224,6 +224,11 @@ static int i2c_nrfx_twim_init(const struct device *dev)
 	return i2c_nrfx_twim_common_init(dev);
 }
 
+static int i2c_nrfx_twim_deinit(const struct device *dev)
+{
+	return i2c_nrfx_twim_common_deinit(dev);
+}
+
 static DEVICE_API(i2c, i2c_nrfx_twim_driver_api) = {
 	.configure = i2c_nrfx_twim_configure,
 	.transfer = i2c_nrfx_twim_transfer,
@@ -280,8 +285,9 @@ static DEVICE_API(i2c, i2c_nrfx_twim_driver_api) = {
 	};								       \
 	PM_DEVICE_DT_DEFINE(I2C(idx), twim_nrfx_pm_action,                     \
 			PM_DEVICE_ISR_SAFE);                                   \
-	I2C_DEVICE_DT_DEFINE(I2C(idx),					       \
+	I2C_DEVICE_DT_DEINIT_DEFINE(I2C(idx),				       \
 		      i2c_nrfx_twim_init,				       \
+		      i2c_nrfx_twim_deinit,				       \
 		      PM_DEVICE_DT_GET(I2C(idx)),			       \
 		      &twim_##idx##_data,				       \
 		      &twim_##idx##z_config,				       \
diff --git a/drivers/i2c/i2c_nrfx_twim_common.c b/drivers/i2c/i2c_nrfx_twim_common.c
index 1539d7c90dd..f7fd0d097b0 100644
--- a/drivers/i2c/i2c_nrfx_twim_common.c
+++ b/drivers/i2c/i2c_nrfx_twim_common.c
@@ -102,18 +102,30 @@ int i2c_nrfx_twim_msg_transfer(const struct device *dev, uint8_t flags, uint8_t
 	return ret;
 }
 
-int twim_nrfx_pm_action(const struct device *dev, enum pm_device_action action)
+void twim_nrfx_pm_resume(const struct device *dev)
 {
 	const struct i2c_nrfx_twim_common_config *config = dev->config;
 
+	(void)pinctrl_apply_state(config->pcfg, PINCTRL_STATE_DEFAULT);
+	nrfx_twim_enable(&config->twim);
+}
+
+void twim_nrfx_pm_suspend(const struct device *dev)
+{
+	const struct i2c_nrfx_twim_common_config *config = dev->config;
+
+	nrfx_twim_disable(&config->twim);
+	(void)pinctrl_apply_state(config->pcfg, PINCTRL_STATE_SLEEP);
+}
+
+int twim_nrfx_pm_action(const struct device *dev, enum pm_device_action action)
+{
 	switch (action) {
 	case PM_DEVICE_ACTION_RESUME:
-		(void)pinctrl_apply_state(config->pcfg, PINCTRL_STATE_DEFAULT);
-		nrfx_twim_enable(&config->twim);
+		twim_nrfx_pm_resume(dev);
 		break;
 	case PM_DEVICE_ACTION_SUSPEND:
-		nrfx_twim_disable(&config->twim);
-		(void)pinctrl_apply_state(config->pcfg, PINCTRL_STATE_SLEEP);
+		twim_nrfx_pm_suspend(dev);
 		break;
 	default:
 		return -ENOTSUP;
@@ -138,3 +150,31 @@ int i2c_nrfx_twim_common_init(const struct device *dev)
 
 	return pm_device_driver_init(dev, twim_nrfx_pm_action);
 }
+
+int i2c_nrfx_twim_common_deinit(const struct device *dev)
+{
+	const struct i2c_nrfx_twim_common_config *config = dev->config;
+#if CONFIG_PM_DEVICE
+	enum pm_device_state state;
+#endif
+
+#if CONFIG_PM_DEVICE
+	/*
+	 * PM must have suspended the device before driver can
+	 * be deinitialized
+	 */
+	(void)pm_device_state_get(dev, &state);
+	if (state != PM_DEVICE_STATE_SUSPENDED &&
+	    state != PM_DEVICE_STATE_OFF) {
+		LOG_ERR("device active");
+		return -EBUSY;
+	}
+#else
+	/* Suspend device */
+	twim_nrfx_pm_suspend(dev);
+#endif
+
+	/* Uninit device hardware */
+	nrfx_twim_uninit(&config->twim);
+	return 0;
+}
diff --git a/drivers/i2c/i2c_nrfx_twim_common.h b/drivers/i2c/i2c_nrfx_twim_common.h
index ba7fa72f019..3c5c82311ba 100644
--- a/drivers/i2c/i2c_nrfx_twim_common.h
+++ b/drivers/i2c/i2c_nrfx_twim_common.h
@@ -43,6 +43,7 @@ struct i2c_nrfx_twim_common_config {
 };
 
 int i2c_nrfx_twim_common_init(const struct device *dev);
+int i2c_nrfx_twim_common_deinit(const struct device *dev);
 int i2c_nrfx_twim_configure(const struct device *dev, uint32_t i2c_config);
 int i2c_nrfx_twim_recover_bus(const struct device *dev);
 int i2c_nrfx_twim_msg_transfer(const struct device *dev, uint8_t flags, uint8_t *buf,
diff --git a/drivers/i2c/i2c_nrfx_twim_rtio.c b/drivers/i2c/i2c_nrfx_twim_rtio.c
index 6a7504dbd62..5eef549ba2e 100644
--- a/drivers/i2c/i2c_nrfx_twim_rtio.c
+++ b/drivers/i2c/i2c_nrfx_twim_rtio.c
@@ -203,7 +203,7 @@ static DEVICE_API(i2c, i2c_nrfx_twim_driver_api) = {
 	.iodev_submit = i2c_nrfx_twim_rtio_submit,
 };
 
-int i2c_nrfx_twim_rtio_init(const struct device *dev)
+static int i2c_nrfx_twim_rtio_init(const struct device *dev)
 {
 	const struct i2c_nrfx_twim_rtio_config *config = dev->config;
 
@@ -211,6 +211,11 @@ int i2c_nrfx_twim_rtio_init(const struct device *dev)
 	return i2c_nrfx_twim_common_init(dev);
 }
 
+static int i2c_nrfx_twim_rtio_deinit(const struct device *dev)
+{
+	return i2c_nrfx_twim_common_deinit(dev);
+}
+
 #define CONCAT_BUF_SIZE(idx)                                                                       \
 	COND_CODE_1(DT_NODE_HAS_PROP(I2C(idx), zephyr_concat_buf_size),                            \
 		    (DT_PROP(I2C(idx), zephyr_concat_buf_size)), (0))
@@ -282,9 +287,10 @@ int i2c_nrfx_twim_rtio_init(const struct device *dev)
 		.ctx = &_i2c##idx##_twim_rtio,                                                     \
 	};                                                                                         \
 	PM_DEVICE_DT_DEFINE(I2C(idx), twim_nrfx_pm_action, PM_DEVICE_ISR_SAFE);                    \
-	I2C_DEVICE_DT_DEFINE(I2C(idx), i2c_nrfx_twim_rtio_init, PM_DEVICE_DT_GET(I2C(idx)),        \
-			     &twim_##idx##z_data, &twim_##idx##z_config, POST_KERNEL,              \
-			     CONFIG_I2C_INIT_PRIORITY, &i2c_nrfx_twim_driver_api);
+	I2C_DEVICE_DT_DEINIT_DEFINE(I2C(idx), i2c_nrfx_twim_rtio_init, i2c_nrfx_twim_rtio_deinit,  \
+			     PM_DEVICE_DT_GET(I2C(idx)), &twim_##idx##z_data,                      \
+			     &twim_##idx##z_config, POST_KERNEL, CONFIG_I2C_INIT_PRIORITY,         \
+			     &i2c_nrfx_twim_driver_api);
 
 #ifdef CONFIG_HAS_HW_NRF_TWIM0
 I2C_NRFX_TWIM_RTIO_DEVICE(0);