diff --git a/dts/arm/nordic/nrf5340_cpuapp_peripherals.dtsi b/dts/arm/nordic/nrf5340_cpuapp_peripherals.dtsi
index 244459a0f16..7021b7eedeb 100644
--- a/dts/arm/nordic/nrf5340_cpuapp_peripherals.dtsi
+++ b/dts/arm/nordic/nrf5340_cpuapp_peripherals.dtsi
@@ -26,6 +26,7 @@ oscillators: clock-controller@4000 {
 		compatible = "nordic,nrf53-hfxo";
 		#clock-cells = <0>;
 		clock-frequency = <DT_FREQ_M(32)>;
+		startup-time-us = <1400>;
 	};
 };
 
diff --git a/dts/bindings/clock/nordic,nrf53-hfxo.yaml b/dts/bindings/clock/nordic,nrf53-hfxo.yaml
index b89cebedac0..f17f4218f65 100644
--- a/dts/bindings/clock/nordic,nrf53-hfxo.yaml
+++ b/dts/bindings/clock/nordic,nrf53-hfxo.yaml
@@ -11,6 +11,20 @@ properties:
   clock-frequency:
     const: 32000000
 
+  startup-time-us:
+    type: int
+    required: true
+    description: |
+      Startup time in microseconds.
+
+      The value can be obtained by first measuring the time between
+      TASKS_HFCLKSTART and EVENTS_HFCLKSTARTED. Then multiply
+      the value by 2 to account for temperature and supply variations.
+
+      Note that the startup time will be longer usual on the first
+      power-up and as such should not be used to determine
+      startup-time-us.
+
   load-capacitors:
     type: string
     enum: