diff --git a/boards/ezurio/bl5340_dvk/CMakeLists.txt b/boards/ezurio/bl5340_dvk/CMakeLists.txt
deleted file mode 100644
index 84b2375f7f8..00000000000
--- a/boards/ezurio/bl5340_dvk/CMakeLists.txt
+++ /dev/null
@@ -1,10 +0,0 @@
-# Copyright (c) 2019 Nordic Semiconductor ASA.
-# Copyright (c) 2021-2023 Laird Connectivity.
-# SPDX-License-Identifier: Apache-2.0
-
-if((CONFIG_BOARD_BL5340_DVK_NRF5340_CPUAPP OR CONFIG_BOARD_BL5340_DVK_NRF5340_CPUAPP_NS)
-   AND CONFIG_BUILD_WITH_TFM)
-  zephyr_library_include_directories(
-    $<TARGET_PROPERTY:tfm,TFM_BINARY_DIR>/api_ns/interface/include
-  )
-endif()
diff --git a/boards/nordic/nrf5340_audio_dk/CMakeLists.txt b/boards/nordic/nrf5340_audio_dk/CMakeLists.txt
index a83f0bf6f09..10ddfc82783 100644
--- a/boards/nordic/nrf5340_audio_dk/CMakeLists.txt
+++ b/boards/nordic/nrf5340_audio_dk/CMakeLists.txt
@@ -4,10 +4,4 @@
 if(CONFIG_BOARD_NRF5340_AUDIO_DK_NRF5340_CPUAPP OR CONFIG_BOARD_NRF5340_AUDIO_DK_NRF5340_CPUAPP_NS)
   zephyr_library()
   zephyr_library_sources(nrf5340_audio_dk_config.c)
-
-  if(CONFIG_BUILD_WITH_TFM)
-    zephyr_library_include_directories(
-      $<TARGET_PROPERTY:tfm,TFM_BINARY_DIR>/api_ns/interface/include
-    )
-  endif()
 endif()
diff --git a/boards/nordic/nrf5340dk/CMakeLists.txt b/boards/nordic/nrf5340dk/CMakeLists.txt
deleted file mode 100644
index 7c4616785f2..00000000000
--- a/boards/nordic/nrf5340dk/CMakeLists.txt
+++ /dev/null
@@ -1,9 +0,0 @@
-# Copyright (c) 2019 Nordic Semiconductor ASA.
-# SPDX-License-Identifier: Apache-2.0
-
-if ((CONFIG_BOARD_NRF5340DK_NRF5340_CPUAPP OR CONFIG_BOARD_NRF5340DK_NRF5340_CPUAPP_NS)
-    AND CONFIG_BUILD_WITH_TFM)
-  zephyr_library_include_directories(
-    $<TARGET_PROPERTY:tfm,TFM_BINARY_DIR>/api_ns/interface/include
-  )
-endif()