diff --git a/scripts/pylib/twister/twisterlib/harness.py b/scripts/pylib/twister/twisterlib/harness.py
index 6c132a10d4e..37e56126289 100644
--- a/scripts/pylib/twister/twisterlib/harness.py
+++ b/scripts/pylib/twister/twisterlib/harness.py
@@ -383,6 +383,10 @@ class Pytest(Harness):
             '--log-cli-format=%(levelname)s: %(message)s'
         ])
 
+        # Use the test timeout as the base timeout for pytest
+        base_timeout = handler.get_test_timeout()
+        command.append(f'--base-timeout={base_timeout}')
+
         if handler.type_str == 'device':
             command.extend(
                 self._generate_parameters_for_hardware(handler)