diff --git a/tests/drivers/adc/adc_api/boards/nucleo_wba65ri.overlay b/tests/drivers/adc/adc_api/boards/nucleo_wba65ri.overlay
new file mode 100644
index 00000000000..4a6e6546c48
--- /dev/null
+++ b/tests/drivers/adc/adc_api/boards/nucleo_wba65ri.overlay
@@ -0,0 +1,34 @@
+/*
+ * SPDX-License-Identifier: Apache-2.0
+ *
+ * Copyright (c) 2025 STMicroelectronics
+ */
+
+/ {
+	zephyr,user {
+		/* adjust channel number according to pinmux in board.dts */
+		io-channels = <&adc4 7>, <&adc4 8>;
+	};
+};
+
+&adc4 {
+	pinctrl-0 = <&adc4_in7_pa2 &adc4_in8_pa1>;
+	#address-cells = <1>;
+	#size-cells = <0>;
+
+	channel@7 {
+		reg = <7>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <ADC_ACQ_TIME_MAX>;
+		zephyr,resolution = <12>;
+	};
+
+	channel@8 {
+		reg = <8>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <ADC_ACQ_TIME_MAX>;
+		zephyr,resolution = <12>;
+	};
+};
diff --git a/tests/drivers/adc/adc_api/testcase.yaml b/tests/drivers/adc/adc_api/testcase.yaml
index 5b99e438ad5..fc0e398ab0f 100644
--- a/tests/drivers/adc/adc_api/testcase.yaml
+++ b/tests/drivers/adc/adc_api/testcase.yaml
@@ -49,6 +49,7 @@ tests:
       - nucleo_u575zi_q
       - nucleo_wb55rg
       - nucleo_wba55cg
+      - nucleo_wba65ri
       - nucleo_wl55jc
       - stm32f3_disco
       - stm32h573i_dk