diff --git a/modules/mbedtls/configs/config-tls-generic.h b/modules/mbedtls/configs/config-tls-generic.h
index 6febce2c60d..383ab44f18b 100644
--- a/modules/mbedtls/configs/config-tls-generic.h
+++ b/modules/mbedtls/configs/config-tls-generic.h
@@ -476,7 +476,7 @@
 #define MBEDTLS_PSA_CRYPTO_C
 #define MBEDTLS_USE_PSA_CRYPTO
 
-#if defined(CONFIG_ARCH_POSIX)
+#if defined(CONFIG_ARCH_POSIX) && !defined(CONFIG_PICOLIBC)
 #define MBEDTLS_PSA_KEY_SLOT_COUNT     64
 #define MBEDTLS_PSA_CRYPTO_STORAGE_C
 #define MBEDTLS_PSA_ITS_FILE_C