diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_bis-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_bis-bt_ll_sw_split.conf
index 0e4a20602b5..c117a556dc1 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_bis-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_bis-bt_ll_sw_split.conf
@@ -77,6 +77,7 @@ CONFIG_BT_CTLR_LLCP_LOCAL_PROC_CTX_BUF_NUM=6
 # ISO Broadcaster Controller
 CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_ADV_PERIODIC=y
+CONFIG_BT_CTLR_SYNC_TRANSFER_SENDER=y
 CONFIG_BT_CTLR_ADV_ISO=y
 CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
 CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
@@ -84,6 +85,7 @@ CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
 # ISO Receive Controller
 CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_SYNC_PERIODIC=y
+CONFIG_BT_CTLR_SYNC_TRANSFER_RECEIVER=y
 CONFIG_BT_CTLR_SYNC_ISO=y
 CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
 CONFIG_BT_CTLR_SYNC_ISO_STREAM_MAX=2
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso-bt_ll_sw_split.conf
index 62a0225b580..f27d32ee080 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso-bt_ll_sw_split.conf
@@ -89,6 +89,7 @@ CONFIG_BT_CTLR_LLCP_LOCAL_PROC_CTX_BUF_NUM=6
 # ISO Broadcaster Controller
 CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_ADV_PERIODIC=y
+CONFIG_BT_CTLR_SYNC_TRANSFER_SENDER=y
 CONFIG_BT_CTLR_ADV_ISO=y
 CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
 CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
@@ -97,6 +98,7 @@ CONFIG_BT_CTLR_ADV_ISO_STREAM_COUNT=2
 # ISO Receive Controller
 CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_SYNC_PERIODIC=y
+CONFIG_BT_CTLR_SYNC_TRANSFER_RECEIVER=y
 CONFIG_BT_CTLR_SYNC_ISO=y
 CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
 CONFIG_BT_CTLR_SYNC_ISO_STREAM_MAX=2
diff --git a/samples/bluetooth/hci_uart/overlay-all-bt_ll_sw_split.conf b/samples/bluetooth/hci_uart/overlay-all-bt_ll_sw_split.conf
index 3b2a93aba04..5885a1cfb28 100644
--- a/samples/bluetooth/hci_uart/overlay-all-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_uart/overlay-all-bt_ll_sw_split.conf
@@ -81,6 +81,7 @@ CONFIG_BT_CTLR_DF_CONN_CTE_REQ=y
 # ISO Broadcaster Controller
 CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_ADV_PERIODIC=y
+CONFIG_BT_CTLR_SYNC_TRANSFER_SENDER=y
 CONFIG_BT_CTLR_ADV_ISO=y
 CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
 CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
@@ -88,6 +89,7 @@ CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
 # ISO Receive Controller
 CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_SYNC_PERIODIC=y
+CONFIG_BT_CTLR_SYNC_TRANSFER_RECEIVER=y
 CONFIG_BT_CTLR_SYNC_ISO=y
 CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
 CONFIG_BT_CTLR_SYNC_ISO_STREAM_MAX=2
diff --git a/samples/bluetooth/hci_uart_3wire/overlay-all-bt_ll_sw_split.conf b/samples/bluetooth/hci_uart_3wire/overlay-all-bt_ll_sw_split.conf
index 548d3a14a2b..df974a21ff4 100644
--- a/samples/bluetooth/hci_uart_3wire/overlay-all-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_uart_3wire/overlay-all-bt_ll_sw_split.conf
@@ -80,6 +80,7 @@ CONFIG_BT_CTLR_DF_CONN_CTE_REQ=y
 # ISO Broadcaster Controller
 CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_ADV_PERIODIC=y
+CONFIG_BT_CTLR_SYNC_TRANSFER_SENDER=y
 CONFIG_BT_CTLR_ADV_ISO=y
 CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
 CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
@@ -87,6 +88,7 @@ CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
 # ISO Receive Controller
 CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_SYNC_PERIODIC=y
+CONFIG_BT_CTLR_SYNC_TRANSFER_RECEIVER=y
 CONFIG_BT_CTLR_SYNC_ISO=y
 CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
 CONFIG_BT_CTLR_SYNC_ISO_STREAM_MAX=2