diff --git a/tests/drivers/spi/spi_loopback/overlay-stm32-spi-16bits-dma-dt-nocache-mem.conf b/tests/drivers/spi/spi_loopback/overlay-stm32-spi-16bits-dma-dt-nocache-mem.conf
deleted file mode 100644
index 4bb96e35f58..00000000000
--- a/tests/drivers/spi/spi_loopback/overlay-stm32-spi-16bits-dma-dt-nocache-mem.conf
+++ /dev/null
@@ -1,14 +0,0 @@
-#
-# Copyright (c) 2023 Graphcore Ltd, All rights reserved.
-#
-# SPDX-License-Identifier: Apache-2.0
-#
-
-# enable DMA mode for SPI loopback test
-CONFIG_SPI_STM32_DMA=y
-CONFIG_SPI_STM32_INTERRUPT=n
-CONFIG_SPI_ASYNC=n
-CONFIG_NOCACHE_MEMORY=n
-CONFIG_DT_DEFINED_NOCACHE=y
-CONFIG_DT_DEFINED_NOCACHE_NAME="SRAM2"
-CONFIG_SPI_LOOPBACK_16BITS_FRAMES=y
diff --git a/tests/drivers/spi/spi_loopback/overlay-stm32-spi-16bits-dma.conf b/tests/drivers/spi/spi_loopback/overlay-stm32-spi-16bits-dma.conf
deleted file mode 100644
index c49efea868a..00000000000
--- a/tests/drivers/spi/spi_loopback/overlay-stm32-spi-16bits-dma.conf
+++ /dev/null
@@ -1,11 +0,0 @@
-#
-# Copyright (c) 2023 Graphcore Ltd, All rights reserved.
-#
-# SPDX-License-Identifier: Apache-2.0
-#
-
-# enable DMA mode for SPI loopback test
-CONFIG_SPI_STM32_DMA=y
-CONFIG_SPI_STM32_INTERRUPT=n
-CONFIG_SPI_ASYNC=n
-CONFIG_SPI_LOOPBACK_16BITS_FRAMES=y
diff --git a/tests/drivers/spi/spi_loopback/overlay-stm32-spi-16bits.conf b/tests/drivers/spi/spi_loopback/overlay-stm32-spi-16bits.conf
deleted file mode 100644
index 00ca04782b4..00000000000
--- a/tests/drivers/spi/spi_loopback/overlay-stm32-spi-16bits.conf
+++ /dev/null
@@ -1,6 +0,0 @@
-#
-# Copyright (c) 2023 Graphcore Ltd, All rights reserved.
-#
-# SPDX-License-Identifier: Apache-2.0
-#
-CONFIG_SPI_LOOPBACK_16BITS_FRAMES=y
diff --git a/tests/drivers/spi/spi_loopback/overlay-stm32-spi-16bits.overlay b/tests/drivers/spi/spi_loopback/overlay-stm32-spi-16bits.overlay
deleted file mode 100644
index b2df25ac860..00000000000
--- a/tests/drivers/spi/spi_loopback/overlay-stm32-spi-16bits.overlay
+++ /dev/null
@@ -1,43 +0,0 @@
-/*
- * Copyright (c) 2023 Graphcore Ltd, All rights reserved.
- *
- * SPDX-License-Identifier: Apache-2.0
- */
-
-/* Set div-q to get test clk freq into acceptable SPI freq range */
-&pll {
-	/delete-property/ div-q;
-	div-q = <8>;
-};
-
-&sram2 {
-	zephyr,memory-attr = < DT_MEM_ARM(ATTR_MPU_RAM_NOCACHE) >;
-};
-
-&spi1 {
-	dmas = <&dmamux1 0 38 (STM32_DMA_PERIPH_TX | STM32_DMA_PRIORITY_HIGH | STM32_DMA_MEM_16BITS | STM32_DMA_PERIPH_16BITS)
-		&dmamux1 1 37 (STM32_DMA_PERIPH_RX | STM32_DMA_PRIORITY_HIGH | STM32_DMA_MEM_16BITS | STM32_DMA_PERIPH_16BITS)>;
-	dma-names = "tx", "rx";
-	slow@0 {
-		compatible = "test-spi-loopback-slow";
-		reg = <0>;
-		spi-max-frequency = <500000>;
-	};
-	fast@0 {
-		compatible = "test-spi-loopback-fast";
-		reg = <0>;
-		spi-max-frequency = <16000000>;
-	};
-};
-
-&dma1 {
-	status = "okay";
-};
-
-&dma2 {
-	status = "okay";
-};
-
-&dmamux1 {
-	status = "okay";
-};
diff --git a/tests/drivers/spi/spi_loopback/testcase.yaml b/tests/drivers/spi/spi_loopback/testcase.yaml
index 6eb098c3dcf..865a561ecc3 100644
--- a/tests/drivers/spi/spi_loopback/testcase.yaml
+++ b/tests/drivers/spi/spi_loopback/testcase.yaml
@@ -51,17 +51,6 @@ tests:
       - robokit1
     integration_platforms:
       - sam_e70_xplained/same70q21
-  drivers.spi.stm32_spi_16bits_frames.loopback:
-    extra_args:
-      - EXTRA_CONF_FILE="overlay-stm32-spi-16bits.conf"
-      - DTC_OVERLAY_FILE="overlay-stm32-spi-16bits.overlay"
-    platform_allow:
-      - nucleo_h743zi
-      - nucleo_h753zi
-      - nucleo_h745zi_q/stm32h745xx/m4
-      - nucleo_h745zi_q/stm32h745xx/m7
-    integration_platforms:
-      - nucleo_h743zi
   drivers.spi.stm32_spi_dma.loopback:
     extra_args: EXTRA_CONF_FILE="overlay-stm32-spi-dma.conf"
     platform_allow:
@@ -98,29 +87,6 @@ tests:
       - nucleo_h745zi_q/stm32h745xx/m7
     integration_platforms:
       - nucleo_h743zi
-  drivers.spi.stm32_spi_16bits_frames_dma.loopback:
-    extra_args:
-      - EXTRA_CONF_FILE="overlay-stm32-spi-16bits-dma.conf"
-      - DTC_OVERLAY_FILE="overlay-stm32-spi-16bits.overlay"
-    platform_allow:
-      - nucleo_h743zi
-      - nucleo_h753zi
-      - nucleo_h745zi_q/stm32h745xx/m4
-      - nucleo_h745zi_q/stm32h745xx/m7
-    integration_platforms:
-      - nucleo_h743zi
-  drivers.spi.stm32_spi_16bits_frames_dma_dt_nocache_mem.loopback:
-    extra_args:
-      - EXTRA_CONF_FILE="overlay-stm32-spi-16bits-dma-dt-nocache-mem.conf"
-      - DTC_OVERLAY_FILE="overlay-stm32-spi-16bits.overlay"
-    filter: CONFIG_CPU_HAS_DCACHE
-    platform_allow:
-      - nucleo_h743zi
-      - nucleo_h753zi
-      - nucleo_h745zi_q/stm32h745xx/m4
-      - nucleo_h745zi_q/stm32h745xx/m7
-    integration_platforms:
-      - nucleo_h743zi
   drivers.spi.stm32_spi_interrupt.loopback:
     extra_args: EXTRA_CONF_FILE="overlay-stm32-spi-interrupt.conf"
     platform_allow: