diff --git a/samples/drivers/mbox/CMakeLists.txt b/samples/drivers/mbox/CMakeLists.txt
index 696b2317aa7..d6bada1a50e 100644
--- a/samples/drivers/mbox/CMakeLists.txt
+++ b/samples/drivers/mbox/CMakeLists.txt
@@ -27,7 +27,6 @@ if(CONFIG_BOARD_NRF5340DK_NRF5340_CPUAPP OR
    CONFIG_BOARD_NRF54H20DK_NRF54H20_CPURAD OR
    CONFIG_BOARD_NRF54L09PDK_NRF54L09_CPUAPP OR
    CONFIG_BOARD_NRF54L15DK_NRF54L15_CPUAPP OR
-   CONFIG_BOARD_NRF54L20PDK_NRF54L20_CPUAPP OR
    CONFIG_BOARD_NRF54LM20DK_NRF54LM20A_CPUAPP OR
    CONFIG_BOARD_STM32H747I_DISCO_STM32H747XX_M7 OR
    CONFIG_BOARD_BL54L15_DVK_NRF54L15_CPUAPP OR
diff --git a/samples/drivers/mbox/Kconfig.sysbuild b/samples/drivers/mbox/Kconfig.sysbuild
index 97402056c14..76d3d240abc 100644
--- a/samples/drivers/mbox/Kconfig.sysbuild
+++ b/samples/drivers/mbox/Kconfig.sysbuild
@@ -21,7 +21,6 @@ config REMOTE_BOARD
 	default "nrf54h20dk/nrf54h20/cpuapp" if "$(BOARD)${BOARD_QUALIFIERS}" = "nrf54h20dk/nrf54h20/cpurad"
 	default "nrf54l09pdk/nrf54l09/cpuflpr" if $(BOARD) = "nrf54l09pdk"
 	default "nrf54l15dk/nrf54l15/cpuflpr" if $(BOARD) = "nrf54l15dk"
-	default "nrf54l20pdk/nrf54l20/cpuflpr" if $(BOARD) = "nrf54l20pdk"
 	default "nrf54lm20dk/nrf54lm20a/cpuflpr" if $(BOARD) = "nrf54lm20dk"
 	default "ophelia4ev/cpuflpr" if $(BOARD) = "ophelia4ev"
 	default "stm32h747i_disco/stm32h747xx/m4" if $(BOARD) = "stm32h747i_disco"
diff --git a/samples/drivers/mbox/boards/nrf54l20pdk_nrf54l20_cpuapp.overlay b/samples/drivers/mbox/boards/nrf54l20pdk_nrf54l20_cpuapp.overlay
deleted file mode 100644
index fd2f23402f5..00000000000
--- a/samples/drivers/mbox/boards/nrf54l20pdk_nrf54l20_cpuapp.overlay
+++ /dev/null
@@ -1,20 +0,0 @@
-/*
- * Copyright 2025 Nordic Semiconductor ASA
- * SPDX-License-Identifier: Apache-2.0
- */
-
-/ {
-	mbox-consumer {
-		compatible = "vnd,mbox-consumer";
-		mboxes = <&cpuapp_vevif_rx 20>, <&cpuapp_vevif_tx 21>;
-		mbox-names = "rx", "tx";
-	};
-};
-
-&cpuapp_vevif_rx {
-	status = "okay";
-};
-
-&cpuapp_vevif_tx {
-	status = "okay";
-};
diff --git a/samples/drivers/mbox/remote/CMakeLists.txt b/samples/drivers/mbox/remote/CMakeLists.txt
index 48df8f0cb5b..c84efec1197 100644
--- a/samples/drivers/mbox/remote/CMakeLists.txt
+++ b/samples/drivers/mbox/remote/CMakeLists.txt
@@ -26,7 +26,6 @@ if(CONFIG_BOARD_NRF5340DK_NRF5340_CPUNET OR
    CONFIG_BOARD_NRF54H20DK_NRF54H20_CPUAPP OR
    CONFIG_BOARD_NRF54L09PDK_NRF54L09_CPUFLPR OR
    CONFIG_BOARD_NRF54L15DK_NRF54L15_CPUFLPR OR
-   CONFIG_BOARD_NRF54L20PDK_NRF54L20_CPUFLPR OR
    CONFIG_BOARD_NRF54LM20DK_NRF54LM20A_CPUFLPR OR
    CONFIG_BOARD_STM32H747I_DISCO_STM32H747XX_M4 OR
    CONFIG_BOARD_BL54L15_DVK_NRF54L15_CPUFLPR OR
diff --git a/samples/drivers/mbox/remote/boards/nrf54l20pdk_nrf54l20_cpuflpr.overlay b/samples/drivers/mbox/remote/boards/nrf54l20pdk_nrf54l20_cpuflpr.overlay
deleted file mode 100644
index ea88120ad06..00000000000
--- a/samples/drivers/mbox/remote/boards/nrf54l20pdk_nrf54l20_cpuflpr.overlay
+++ /dev/null
@@ -1,24 +0,0 @@
-/*
- * Copyright 2025 Nordic Semiconductor ASA
- * SPDX-License-Identifier: Apache-2.0
- */
-
-/ {
-	mbox-consumer {
-		compatible = "vnd,mbox-consumer";
-		mboxes = <&cpuflpr_vevif_rx 21>, <&cpuflpr_vevif_tx 20>;
-		mbox-names = "rx", "tx";
-	};
-};
-
-&cpuflpr_vevif_rx {
-	status = "okay";
-};
-
-&cpuflpr_vevif_tx {
-	status = "okay";
-};
-
-&uart30 {
-	/delete-property/ hw-flow-control;
-};
diff --git a/samples/drivers/mbox/sample.yaml b/samples/drivers/mbox/sample.yaml
index 1aee77b1c67..809d5e091dc 100644
--- a/samples/drivers/mbox/sample.yaml
+++ b/samples/drivers/mbox/sample.yaml
@@ -12,7 +12,6 @@ tests:
       - nrf54h20dk/nrf54h20/cpurad
       - nrf54l09pdk/nrf54l09/cpuapp
       - nrf54l15dk/nrf54l15/cpuapp
-      - nrf54l20pdk/nrf54l20/cpuapp
       - nrf54lm20dk/nrf54lm20a/cpuapp
     integration_platforms:
       - nrf5340bsim/nrf5340/cpuapp
@@ -78,7 +77,6 @@ tests:
     platform_allow:
       - nrf54l09pdk/nrf54l09/cpuapp
       - nrf54l15dk/nrf54l15/cpuapp
-      - nrf54l20pdk/nrf54l20/cpuapp
       - nrf54lm20dk/nrf54lm20a/cpuapp
     integration_platforms:
       - nrf54l15dk/nrf54l15/cpuapp
@@ -96,7 +94,6 @@ tests:
     platform_allow:
       - nrf54l09pdk/nrf54l09/cpuapp
       - nrf54l15dk/nrf54l15/cpuapp
-      - nrf54l20pdk/nrf54l20/cpuapp
       - nrf54lm20dk/nrf54lm20a/cpuapp
     integration_platforms:
       - nrf54l15dk/nrf54l15/cpuapp
@@ -117,7 +114,6 @@ tests:
     platform_allow:
       - nrf54l09pdk/nrf54l09/cpuapp
       - nrf54l15dk/nrf54l15/cpuapp
-      - nrf54l20pdk/nrf54l20/cpuapp
       - nrf54lm20dk/nrf54lm20a/cpuapp
     integration_platforms:
       - nrf54l15dk/nrf54l15/cpuapp
diff --git a/samples/drivers/mbox/sysbuild.cmake b/samples/drivers/mbox/sysbuild.cmake
index 31802733514..0644500ca48 100644
--- a/samples/drivers/mbox/sysbuild.cmake
+++ b/samples/drivers/mbox/sysbuild.cmake
@@ -14,6 +14,7 @@ ExternalZephyrProject_Add(
 	APPLICATION ${REMOTE_APP}
 	SOURCE_DIR  ${APP_DIR}/${REMOTE_APP}
 	BOARD       ${SB_CONFIG_REMOTE_BOARD}
+	BOARD_REVISION ${BOARD_REVISION}
 )
 
 native_simulator_set_child_images(${DEFAULT_IMAGE} ${REMOTE_APP})