diff --git a/tests/drivers/spi/spi_loopback/boards/atsame54_xpro.conf b/tests/drivers/spi/spi_loopback/boards/atsame54_xpro.conf
new file mode 100644
index 00000000000..8497667443d
--- /dev/null
+++ b/tests/drivers/spi/spi_loopback/boards/atsame54_xpro.conf
@@ -0,0 +1,2 @@
+CONFIG_SPI_LOOPBACK_DRV_NAME="SPI_0"
+CONFIG_SPI_LOOPBACK_CS_GPIO=n