diff --git a/boards/ti/lp_em_cc2340r5/lp_em_cc2340r5-pinctrl.dtsi b/boards/ti/lp_em_cc2340r5/lp_em_cc2340r5-pinctrl.dtsi
index 1d929cfa6ac..eb19f89888f 100644
--- a/boards/ti/lp_em_cc2340r5/lp_em_cc2340r5-pinctrl.dtsi
+++ b/boards/ti/lp_em_cc2340r5/lp_em_cc2340r5-pinctrl.dtsi
@@ -13,9 +13,32 @@
 		pinmux = <20 DIO20_UART0_TXD>;
 		bias-disable;
 	};
+
 	uart0_rx_default: uart0_rx_default {
 		pinmux = <22 DIO22_UART0_RXD>;
 		bias-disable;
 		input-enable;
 	};
+
+	/* SPI0 */
+	spi0_sck_default: spi0_sck_default {
+		pinmux = <18 DIO18_SPI0_SCLK>;
+		bias-disable;
+	};
+
+	spi0_mosi_default: spi0_mosi_default {
+		pinmux = <13 DIO13_SPI0_MOSI>;
+		bias-disable;
+	};
+
+	spi0_miso_default: spi0_miso_default {
+		pinmux = <12 DIO12_SPI0_MISO>;
+		bias-disable;
+		input-enable;
+	};
+
+	spi0_cs_default: spi0_cs_default {
+		pinmux = <11 DIO11_SPI0_CSN>;
+		bias-disable;
+	};
 };
diff --git a/boards/ti/lp_em_cc2340r5/lp_em_cc2340r5.dts b/boards/ti/lp_em_cc2340r5/lp_em_cc2340r5.dts
index 698820d81c3..90288e5ba8f 100644
--- a/boards/ti/lp_em_cc2340r5/lp_em_cc2340r5.dts
+++ b/boards/ti/lp_em_cc2340r5/lp_em_cc2340r5.dts
@@ -81,3 +81,12 @@
 &dma {
 	status = "okay";
 };
+
+&spi0 {
+	status = "okay";
+	pinctrl-0 = <&spi0_sck_default
+		     &spi0_mosi_default
+		     &spi0_miso_default
+		     &spi0_cs_default>;
+	pinctrl-names = "default";
+};