diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_bis-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_bis-bt_ll_sw_split.conf
index c117a556dc1..a7487118694 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_bis-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_bis-bt_ll_sw_split.conf
@@ -5,13 +5,10 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_IPC_SERVICE_BACKEND_RPMSG_WQ_STACK_SIZE=512
 CONFIG_HEAP_MEM_POOL_SIZE=8192
 
 CONFIG_BT=y
-CONFIG_BT_OBSERVER=y
 CONFIG_BT_HCI_RAW=y
-CONFIG_BT_MAX_CONN=2
 
 # Workaround: Unable to allocate command buffer when using K_NO_WAIT since
 # Host number of completed commands does not follow normal flow control.
@@ -24,11 +21,16 @@ CONFIG_BT_BUF_ACL_RX_SIZE=255
 CONFIG_BT_BUF_ACL_TX_SIZE=251
 CONFIG_BT_BUF_CMD_TX_SIZE=255
 
-# Host features
+# Host and Controller common dependencies
+CONFIG_BT_BROADCASTER=y
+CONFIG_BT_OBSERVER=y
 CONFIG_BT_EXT_ADV=y
 CONFIG_BT_PER_ADV=y
 CONFIG_BT_PER_ADV_SYNC=y
 CONFIG_BT_PER_ADV_SYNC_MAX=2
+CONFIG_BT_CENTRAL=y
+CONFIG_BT_PERIPHERAL=y
+CONFIG_BT_MAX_CONN=2
 
 # Broadcast and Connected ISO
 CONFIG_BT_ISO_BROADCASTER=y
@@ -37,7 +39,9 @@ CONFIG_BT_ISO_CENTRAL=n
 CONFIG_BT_ISO_PERIPHERAL=n
 
 # ISO Streams
-CONFIG_BT_ISO_MAX_CHAN=2
+CONFIG_BT_ISO_MAX_CHAN=4
+CONFIG_BT_ISO_TX_BUF_COUNT=1
+CONFIG_BT_ISO_RX_BUF_COUNT=1
 
 # Controller
 CONFIG_BT_LL_SW_SPLIT=y
@@ -79,21 +83,25 @@ CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_ADV_PERIODIC=y
 CONFIG_BT_CTLR_SYNC_TRANSFER_SENDER=y
 CONFIG_BT_CTLR_ADV_ISO=y
-CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
+CONFIG_BT_CTLR_ADV_ISO_SET=2
+CONFIG_BT_CTLR_ADV_ISO_STREAM_COUNT=4
 CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
+CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
 
 # ISO Receive Controller
 CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_SYNC_PERIODIC=y
 CONFIG_BT_CTLR_SYNC_TRANSFER_RECEIVER=y
 CONFIG_BT_CTLR_SYNC_ISO=y
-CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
+CONFIG_BT_CTLR_SCAN_SYNC_ISO_SET=1
+CONFIG_BT_CTLR_SYNC_ISO_STREAM_COUNT=2
 CONFIG_BT_CTLR_SYNC_ISO_STREAM_MAX=2
+CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
 
 # ISO Transmissions
-CONFIG_BT_CTLR_ISO_TX_BUFFERS=8
+CONFIG_BT_CTLR_ISOAL_SOURCES=4
+CONFIG_BT_CTLR_ISO_TX_BUFFERS=12
 CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
-CONFIG_BT_CTLR_ISOAL_SOURCES=2
 
 # ISO Receptions
 CONFIG_BT_CTLR_ISO_RX_BUFFERS=8
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_cis-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_cis-bt_ll_sw_split.conf
index af142bf7dc6..f7e1f8fc673 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_cis-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_cis-bt_ll_sw_split.conf
@@ -5,12 +5,10 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_IPC_SERVICE_BACKEND_RPMSG_WQ_STACK_SIZE=512
 CONFIG_HEAP_MEM_POOL_SIZE=8192
 
 CONFIG_BT=y
 CONFIG_BT_HCI_RAW=y
-CONFIG_BT_MAX_CONN=2
 
 # Workaround: Unable to allocate command buffer when using K_NO_WAIT since
 # Host number of completed commands does not follow normal flow control.
@@ -23,12 +21,15 @@ CONFIG_BT_BUF_ACL_RX_SIZE=255
 CONFIG_BT_BUF_ACL_TX_SIZE=251
 CONFIG_BT_BUF_CMD_TX_SIZE=255
 
-# Host features
+# Host and Controller common dependencies
+CONFIG_BT_BROADCASTER=y
+CONFIG_BT_OBSERVER=y
 CONFIG_BT_EXT_ADV=y
-CONFIG_BT_PERIPHERAL=y
-CONFIG_BT_CENTRAL=y
 CONFIG_BT_PER_ADV=n
 CONFIG_BT_PER_ADV_SYNC=n
+CONFIG_BT_CENTRAL=y
+CONFIG_BT_PERIPHERAL=y
+CONFIG_BT_MAX_CONN=3
 
 # Broadcast and Connected ISO
 CONFIG_BT_ISO_BROADCASTER=n
@@ -37,7 +38,9 @@ CONFIG_BT_ISO_CENTRAL=y
 CONFIG_BT_ISO_PERIPHERAL=y
 
 # ISO Streams
-CONFIG_BT_ISO_MAX_CHAN=2
+CONFIG_BT_ISO_MAX_CHAN=4
+CONFIG_BT_ISO_TX_BUF_COUNT=1
+CONFIG_BT_ISO_RX_BUF_COUNT=1
 
 # Controller
 CONFIG_BT_LL_SW_SPLIT=y
@@ -79,13 +82,17 @@ CONFIG_BT_CTLR_LLCP_LOCAL_PROC_CTX_BUF_NUM=6
 # ISO Connection Oriented
 CONFIG_BT_CTLR_CENTRAL_ISO=y
 CONFIG_BT_CTLR_PERIPHERAL_ISO=y
+CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
+CONFIG_BT_CTLR_CONN_ISO_GROUPS=1
+CONFIG_BT_CTLR_CONN_ISO_STREAMS_PER_GROUP=2
 CONFIG_BT_CTLR_CONN_ISO_SDU_LEN_MAX=247
 CONFIG_BT_CTLR_CONN_ISO_PDU_LEN_MAX=251
+CONFIG_BT_CTLR_CONN_ISO_LOW_LATENCY_POLICY=y
 
 # ISO Transmissions
-CONFIG_BT_CTLR_ISO_TX_BUFFERS=8
-CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
 CONFIG_BT_CTLR_ISOAL_SOURCES=2
+CONFIG_BT_CTLR_ISO_TX_BUFFERS=12
+CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
 
 # ISO Receptions
 CONFIG_BT_CTLR_ISO_RX_BUFFERS=8
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_df-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_df-bt_ll_sw_split.conf
index 403501a5810..b183a4c9825 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_df-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_df-bt_ll_sw_split.conf
@@ -5,12 +5,10 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_IPC_SERVICE_BACKEND_RPMSG_WQ_STACK_SIZE=512
 CONFIG_HEAP_MEM_POOL_SIZE=8192
 
 CONFIG_BT=y
 CONFIG_BT_HCI_RAW=y
-CONFIG_BT_MAX_CONN=2
 
 # Workaround: Unable to allocate command buffer when using K_NO_WAIT since
 # Host number of completed commands does not follow normal flow control.
@@ -23,11 +21,16 @@ CONFIG_BT_BUF_ACL_RX_SIZE=255
 CONFIG_BT_BUF_ACL_TX_SIZE=251
 CONFIG_BT_BUF_CMD_TX_SIZE=255
 
-# Host features
+# Host and Controller common dependencies
+CONFIG_BT_BROADCASTER=y
+CONFIG_BT_OBSERVER=y
 CONFIG_BT_EXT_ADV=y
 CONFIG_BT_PER_ADV=y
 CONFIG_BT_PER_ADV_SYNC=y
 CONFIG_BT_PER_ADV_SYNC_MAX=2
+CONFIG_BT_CENTRAL=y
+CONFIG_BT_PERIPHERAL=y
+CONFIG_BT_MAX_CONN=2
 
 # Controller
 CONFIG_BT_LL_SW_SPLIT=y
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso-bt_ll_sw_split.conf
index f27d32ee080..925eac8e34e 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso-bt_ll_sw_split.conf
@@ -5,7 +5,6 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_IPC_SERVICE_BACKEND_RPMSG_WQ_STACK_SIZE=512
 CONFIG_HEAP_MEM_POOL_SIZE=8192
 CONFIG_CBPRINTF_REDUCED_INTEGRAL=y
 
@@ -13,10 +12,7 @@ CONFIG_ISR_TABLES_LOCAL_DECLARATION=y
 CONFIG_LTO=y
 
 CONFIG_BT=y
-CONFIG_BT_PERIPHERAL=y
-CONFIG_BT_CENTRAL=y
 CONFIG_BT_HCI_RAW=y
-CONFIG_BT_MAX_CONN=3
 
 # Workaround: Unable to allocate command buffer when using K_NO_WAIT since
 # Host number of completed commands does not follow normal flow control.
@@ -29,11 +25,16 @@ CONFIG_BT_BUF_ACL_RX_SIZE=255
 CONFIG_BT_BUF_ACL_TX_SIZE=251
 CONFIG_BT_BUF_CMD_TX_SIZE=255
 
-# Host features
+# Host and Controller common dependencies
+CONFIG_BT_BROADCASTER=y
+CONFIG_BT_OBSERVER=y
 CONFIG_BT_EXT_ADV=y
 CONFIG_BT_PER_ADV=y
 CONFIG_BT_PER_ADV_SYNC=y
 CONFIG_BT_PER_ADV_SYNC_MAX=2
+CONFIG_BT_CENTRAL=y
+CONFIG_BT_PERIPHERAL=y
+CONFIG_BT_MAX_CONN=3
 
 # Broadcast and Connected ISO
 CONFIG_BT_ISO_BROADCASTER=y
@@ -42,7 +43,11 @@ CONFIG_BT_ISO_CENTRAL=y
 CONFIG_BT_ISO_PERIPHERAL=y
 
 # ISO Streams
+CONFIG_BT_ISO_TX_MTU=310
+CONFIG_BT_ISO_RX_MTU=310
 CONFIG_BT_ISO_MAX_CHAN=4
+CONFIG_BT_ISO_TX_BUF_COUNT=1
+CONFIG_BT_ISO_RX_BUF_COUNT=1
 
 # Controller
 CONFIG_BT_LL_SW_SPLIT=y
@@ -71,8 +76,12 @@ CONFIG_BT_CTLR_ADV_DATA_BUF_MAX=6
 
 # Increase the below to receive interleaved advertising chains
 CONFIG_BT_CTLR_SCAN_AUX_SET=1
+# CONFIG_BT_CTLR_SCAN_AUX_USE_CHAINS=y
+# CONFIG_BT_CTLR_SCAN_AUX_CHAIN_COUNT=1
 
 CONFIG_BT_CTLR_ADV_RESERVE_MAX=n
+CONFIG_BT_CTLR_ADV_ISO_RESERVE_MAX=y
+CONFIG_BT_CTLR_SYNC_ISO_RESERVE_MAX=n
 CONFIG_BT_CTLR_CENTRAL_RESERVE_MAX=n
 CONFIG_BT_CTLR_PERIPHERAL_RESERVE_MAX=n
 CONFIG_BT_CTLR_PERIPHERAL_ISO_RESERVE_MAX=n
@@ -91,38 +100,42 @@ CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_ADV_PERIODIC=y
 CONFIG_BT_CTLR_SYNC_TRANSFER_SENDER=y
 CONFIG_BT_CTLR_ADV_ISO=y
-CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
+CONFIG_BT_CTLR_ADV_ISO_SET=2
+CONFIG_BT_CTLR_ADV_ISO_STREAM_COUNT=4
 CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
-CONFIG_BT_CTLR_ADV_ISO_STREAM_COUNT=2
+CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
 
 # ISO Receive Controller
 CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_SYNC_PERIODIC=y
 CONFIG_BT_CTLR_SYNC_TRANSFER_RECEIVER=y
 CONFIG_BT_CTLR_SYNC_ISO=y
-CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
+CONFIG_BT_CTLR_SCAN_SYNC_ISO_SET=1
+CONFIG_BT_CTLR_SYNC_ISO_STREAM_COUNT=2
 CONFIG_BT_CTLR_SYNC_ISO_STREAM_MAX=2
+CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
 
 # ISO Connection Oriented
 CONFIG_BT_CTLR_CENTRAL_ISO=y
 CONFIG_BT_CTLR_PERIPHERAL_ISO=y
 CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
+CONFIG_BT_CTLR_CONN_ISO_GROUPS=1
 CONFIG_BT_CTLR_CONN_ISO_STREAMS_PER_GROUP=2
 CONFIG_BT_CTLR_CONN_ISO_SDU_LEN_MAX=247
 CONFIG_BT_CTLR_CONN_ISO_PDU_LEN_MAX=251
 CONFIG_BT_CTLR_CONN_ISO_LOW_LATENCY_POLICY=y
 
 # ISO Transmissions
-CONFIG_BT_ISO_TX_MTU=310
-CONFIG_BT_ISO_TX_BUF_COUNT=4
-CONFIG_BT_CTLR_ISO_TX_BUFFERS=8
+CONFIG_BT_CTLR_ISOAL_SOURCES=4
+CONFIG_BT_CTLR_ISO_TX_BUFFERS=12
 CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
-CONFIG_BT_CTLR_ISOAL_SOURCES=2
 
 # ISO Receptions
-CONFIG_BT_ISO_RX_MTU=310
-CONFIG_BT_CTLR_ISO_RX_BUFFERS=8
 CONFIG_BT_CTLR_ISOAL_SINKS=2
+CONFIG_BT_CTLR_ISO_RX_BUFFERS=8
 
 # Tx Power Dynamic Control
 CONFIG_BT_CTLR_TX_PWR_DYNAMIC_CONTROL=y
+
+# Ignore HCI ISO data Tx sequence numbers
+# CONFIG_BT_CTLR_ISOAL_PSN_IGNORE=y
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_broadcast-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_broadcast-bt_ll_sw_split.conf
index 05645663bc4..67c5854c9c0 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_broadcast-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_broadcast-bt_ll_sw_split.conf
@@ -5,7 +5,6 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_IPC_SERVICE_BACKEND_RPMSG_WQ_STACK_SIZE=512
 CONFIG_HEAP_MEM_POOL_SIZE=8192
 
 CONFIG_BT=y
@@ -15,26 +14,30 @@ CONFIG_BT_HCI_RAW=y
 # Host number of completed commands does not follow normal flow control.
 CONFIG_BT_BUF_CMD_TX_COUNT=10
 
-# Host
+# Host and Controller common dependencies
 CONFIG_BT_BROADCASTER=y
-CONFIG_BT_PERIPHERAL=n
 CONFIG_BT_OBSERVER=n
-CONFIG_BT_CENTRAL=n
 CONFIG_BT_EXT_ADV=y
 CONFIG_BT_PER_ADV=y
 CONFIG_BT_ISO_BROADCASTER=y
-CONFIG_BT_ISO_MAX_CHAN=2
+CONFIG_BT_ISO_MAX_CHAN=4
+CONFIG_BT_ISO_TX_BUF_COUNT=1
+CONFIG_BT_CENTRAL=n
+CONFIG_BT_PERIPHERAL=n
 
 # ISO Broadcast Controller
 CONFIG_BT_LL_SW_SPLIT=y
 CONFIG_BT_CTLR_ADV_PERIODIC=y
 CONFIG_BT_CTLR_ADV_DATA_LEN_MAX=191
 CONFIG_BT_CTLR_ADV_ISO=y
-CONFIG_BT_CTLR_ISO_TX_BUFFERS=16
-CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
-CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
+CONFIG_BT_CTLR_ADV_ISO_SET=2
+CONFIG_BT_CTLR_ADV_ISO_STREAM_COUNT=4
 CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
-CONFIG_BT_CTLR_ISOAL_SOURCES=2
+CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
+CONFIG_BT_CTLR_ISOAL_SOURCES=4
+
+CONFIG_BT_CTLR_ISO_TX_BUFFERS=12
+CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
 
 CONFIG_BT_CTLR_ADVANCED_FEATURES=y
 CONFIG_BT_CTLR_ADV_RESERVE_MAX=n
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_central-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_central-bt_ll_sw_split.conf
index 56e80860f32..505b9d352ba 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_central-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_central-bt_ll_sw_split.conf
@@ -5,7 +5,6 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_IPC_SERVICE_BACKEND_RPMSG_WQ_STACK_SIZE=512
 CONFIG_HEAP_MEM_POOL_SIZE=8192
 
 CONFIG_BT=y
@@ -20,21 +19,24 @@ CONFIG_BT_BUF_ACL_RX_SIZE=255
 CONFIG_BT_BUF_ACL_TX_SIZE=251
 CONFIG_BT_BUF_CMD_TX_SIZE=255
 
-# Host
+# Host and Controller common dependencies
 CONFIG_BT_BROADCASTER=n
-CONFIG_BT_PERIPHERAL=n
 CONFIG_BT_OBSERVER=y
-CONFIG_BT_CENTRAL=y
 CONFIG_BT_EXT_ADV=y
 CONFIG_BT_PER_ADV=n
 CONFIG_BT_PER_ADV_SYNC=n
 CONFIG_BT_ISO_BROADCASTER=n
 CONFIG_BT_ISO_SYNC_RECEIVER=n
+CONFIG_BT_CENTRAL=y
+CONFIG_BT_PERIPHERAL=n
+CONFIG_BT_MAX_CONN=2
 CONFIG_BT_ISO_CENTRAL=y
 CONFIG_BT_ISO_PERIPHERAL=n
 
 # ISO Streams
 CONFIG_BT_ISO_MAX_CHAN=2
+CONFIG_BT_ISO_TX_BUF_COUNT=1
+CONFIG_BT_ISO_RX_BUF_COUNT=1
 
 # Controller
 CONFIG_BT_LL_SW_SPLIT=y
@@ -44,17 +46,21 @@ CONFIG_BT_CTLR_SCAN_DATA_LEN_MAX=191
 # ISO Connection Oriented
 CONFIG_BT_CTLR_CENTRAL_ISO=y
 CONFIG_BT_CTLR_PERIPHERAL_ISO=n
+CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
+CONFIG_BT_CTLR_CONN_ISO_GROUPS=1
+CONFIG_BT_CTLR_CONN_ISO_STREAMS_PER_GROUP=2
 CONFIG_BT_CTLR_CONN_ISO_SDU_LEN_MAX=247
 CONFIG_BT_CTLR_CONN_ISO_PDU_LEN_MAX=251
+CONFIG_BT_CTLR_CONN_ISO_LOW_LATENCY_POLICY=y
 
 # ISO Transmissions
-CONFIG_BT_CTLR_ISO_TX_BUFFERS=16
-CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
 CONFIG_BT_CTLR_ISOAL_SOURCES=2
+CONFIG_BT_CTLR_ISO_TX_BUFFERS=12
+CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
 
 # ISO Receptions
-CONFIG_BT_CTLR_ISO_RX_BUFFERS=16
 CONFIG_BT_CTLR_ISOAL_SINKS=2
+CONFIG_BT_CTLR_ISO_RX_BUFFERS=8
 
 # Controller with minimum ACL event length and unreserved scanning
 CONFIG_BT_CTLR_ADVANCED_FEATURES=y
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_peripheral-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_peripheral-bt_ll_sw_split.conf
index b72aaa624f1..05c9a9d3903 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_peripheral-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_peripheral-bt_ll_sw_split.conf
@@ -5,7 +5,6 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_IPC_SERVICE_BACKEND_RPMSG_WQ_STACK_SIZE=512
 CONFIG_HEAP_MEM_POOL_SIZE=8192
 
 CONFIG_BT=y
@@ -20,21 +19,24 @@ CONFIG_BT_BUF_ACL_RX_SIZE=255
 CONFIG_BT_BUF_ACL_TX_SIZE=251
 CONFIG_BT_BUF_CMD_TX_SIZE=255
 
-# Host
+# Host and Controller common dependencies
 CONFIG_BT_BROADCASTER=y
-CONFIG_BT_PERIPHERAL=y
 CONFIG_BT_OBSERVER=n
-CONFIG_BT_CENTRAL=n
 CONFIG_BT_EXT_ADV=y
 CONFIG_BT_PER_ADV=n
 CONFIG_BT_PER_ADV_SYNC=n
 CONFIG_BT_ISO_BROADCASTER=n
 CONFIG_BT_ISO_SYNC_RECEIVER=n
+CONFIG_BT_CENTRAL=n
+CONFIG_BT_PERIPHERAL=y
+CONFIG_BT_MAX_CONN=1
 CONFIG_BT_ISO_CENTRAL=n
 CONFIG_BT_ISO_PERIPHERAL=y
 
 # ISO Streams
 CONFIG_BT_ISO_MAX_CHAN=2
+CONFIG_BT_ISO_TX_BUF_COUNT=1
+CONFIG_BT_ISO_RX_BUF_COUNT=1
 
 # Controller
 CONFIG_BT_LL_SW_SPLIT=y
@@ -44,17 +46,21 @@ CONFIG_BT_CTLR_ADV_DATA_LEN_MAX=191
 # ISO Connection Oriented
 CONFIG_BT_CTLR_CENTRAL_ISO=n
 CONFIG_BT_CTLR_PERIPHERAL_ISO=y
+CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
+CONFIG_BT_CTLR_CONN_ISO_GROUPS=1
+CONFIG_BT_CTLR_CONN_ISO_STREAMS_PER_GROUP=2
 CONFIG_BT_CTLR_CONN_ISO_SDU_LEN_MAX=247
 CONFIG_BT_CTLR_CONN_ISO_PDU_LEN_MAX=251
+CONFIG_BT_CTLR_CONN_ISO_LOW_LATENCY_POLICY=y
 
 # ISO Transmissions
-CONFIG_BT_CTLR_ISO_TX_BUFFERS=16
-CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
 CONFIG_BT_CTLR_ISOAL_SOURCES=2
+CONFIG_BT_CTLR_ISO_TX_BUFFERS=12
+CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
 
 # ISO Receptions
-CONFIG_BT_CTLR_ISO_RX_BUFFERS=16
 CONFIG_BT_CTLR_ISOAL_SINKS=2
+CONFIG_BT_CTLR_ISO_RX_BUFFERS=8
 
 CONFIG_BT_CTLR_ADVANCED_FEATURES=y
 CONFIG_BT_CTLR_ADV_RESERVE_MAX=n
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_receive-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_receive-bt_ll_sw_split.conf
index cc3b5adecdd..51ca53e83f2 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_receive-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_receive-bt_ll_sw_split.conf
@@ -5,7 +5,6 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_IPC_SERVICE_BACKEND_RPMSG_WQ_STACK_SIZE=512
 CONFIG_HEAP_MEM_POOL_SIZE=8192
 
 CONFIG_BT=y
@@ -15,22 +14,24 @@ CONFIG_BT_HCI_RAW=y
 # Host number of completed commands does not follow normal flow control.
 CONFIG_BT_BUF_CMD_TX_COUNT=10
 
-# Host
+# Host and Controller common dependencies
 CONFIG_BT_BROADCASTER=n
-CONFIG_BT_PERIPHERAL=n
 CONFIG_BT_OBSERVER=y
-CONFIG_BT_CENTRAL=n
 CONFIG_BT_EXT_ADV=y
 CONFIG_BT_PER_ADV_SYNC=y
 CONFIG_BT_ISO_SYNC_RECEIVER=y
-CONFIG_BT_ISO_MAX_CHAN=2
+CONFIG_BT_ISO_MAX_CHAN=4
+CONFIG_BT_CENTRAL=n
+CONFIG_BT_PERIPHERAL=n
 
 # ISO Receive Controller
 CONFIG_BT_LL_SW_SPLIT=y
 CONFIG_BT_CTLR_SYNC_PERIODIC=y
 CONFIG_BT_CTLR_SCAN_DATA_LEN_MAX=191
 CONFIG_BT_CTLR_SYNC_ISO=y
-CONFIG_BT_CTLR_ISO_RX_BUFFERS=16
-CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
+CONFIG_BT_CTLR_SCAN_SYNC_ISO_SET=1
+CONFIG_BT_CTLR_SYNC_ISO_STREAM_COUNT=2
 CONFIG_BT_CTLR_SYNC_ISO_STREAM_MAX=2
+CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
 CONFIG_BT_CTLR_ISOAL_SINKS=2
+CONFIG_BT_CTLR_ISO_RX_BUFFERS=8
diff --git a/samples/bluetooth/hci_uart/overlay-all-bt_ll_sw_split.conf b/samples/bluetooth/hci_uart/overlay-all-bt_ll_sw_split.conf
index 5885a1cfb28..a4054de0934 100644
--- a/samples/bluetooth/hci_uart/overlay-all-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_uart/overlay-all-bt_ll_sw_split.conf
@@ -10,8 +10,8 @@ CONFIG_BT_EXT_ADV=y
 CONFIG_BT_PER_ADV=y
 CONFIG_BT_PER_ADV_SYNC=y
 CONFIG_BT_PER_ADV_SYNC_MAX=2
-CONFIG_BT_PERIPHERAL=y
 CONFIG_BT_CENTRAL=y
+CONFIG_BT_PERIPHERAL=y
 
 # Broadcast and Connected ISO
 CONFIG_BT_ISO_BROADCASTER=y
@@ -20,7 +20,11 @@ CONFIG_BT_ISO_CENTRAL=y
 CONFIG_BT_ISO_PERIPHERAL=y
 
 # ISO Streams
-CONFIG_BT_ISO_MAX_CHAN=2
+CONFIG_BT_ISO_TX_MTU=310
+CONFIG_BT_ISO_RX_MTU=310
+CONFIG_BT_ISO_MAX_CHAN=4
+CONFIG_BT_ISO_TX_BUF_COUNT=1
+CONFIG_BT_ISO_RX_BUF_COUNT=1
 
 # Controller
 CONFIG_BT_LL_SW_SPLIT=y
@@ -49,9 +53,16 @@ CONFIG_BT_CTLR_ADV_DATA_BUF_MAX=6
 
 # Increase the below to receive interleaved advertising chains
 CONFIG_BT_CTLR_SCAN_AUX_SET=1
+# CONFIG_BT_CTLR_SCAN_AUX_USE_CHAINS=y
+# CONFIG_BT_CTLR_SCAN_AUX_CHAIN_COUNT=1
 
 CONFIG_BT_CTLR_ADV_RESERVE_MAX=n
+CONFIG_BT_CTLR_ADV_ISO_RESERVE_MAX=y
+CONFIG_BT_CTLR_SYNC_ISO_RESERVE_MAX=n
 CONFIG_BT_CTLR_CENTRAL_RESERVE_MAX=n
+CONFIG_BT_CTLR_PERIPHERAL_RESERVE_MAX=n
+CONFIG_BT_CTLR_PERIPHERAL_ISO_RESERVE_MAX=n
+CONFIG_BT_CTLR_EVENT_OVERHEAD_RESERVE_MAX=y
 CONFIG_BT_CTLR_SLOT_RESERVATION_UPDATE=n
 CONFIG_BT_CTLR_SCAN_UNRESERVED=y
 CONFIG_BT_TICKER_NEXT_SLOT_GET_MATCH=y
@@ -83,31 +94,42 @@ CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_ADV_PERIODIC=y
 CONFIG_BT_CTLR_SYNC_TRANSFER_SENDER=y
 CONFIG_BT_CTLR_ADV_ISO=y
-CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
+CONFIG_BT_CTLR_ADV_ISO_SET=2
+CONFIG_BT_CTLR_ADV_ISO_STREAM_COUNT=4
 CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
+CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
 
 # ISO Receive Controller
 CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_SYNC_PERIODIC=y
 CONFIG_BT_CTLR_SYNC_TRANSFER_RECEIVER=y
 CONFIG_BT_CTLR_SYNC_ISO=y
-CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
+CONFIG_BT_CTLR_SCAN_SYNC_ISO_SET=1
+CONFIG_BT_CTLR_SYNC_ISO_STREAM_COUNT=2
 CONFIG_BT_CTLR_SYNC_ISO_STREAM_MAX=2
+CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
 
 # ISO Connection Oriented
 CONFIG_BT_CTLR_CENTRAL_ISO=y
 CONFIG_BT_CTLR_PERIPHERAL_ISO=y
+CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
+CONFIG_BT_CTLR_CONN_ISO_GROUPS=1
+CONFIG_BT_CTLR_CONN_ISO_STREAMS_PER_GROUP=2
 CONFIG_BT_CTLR_CONN_ISO_SDU_LEN_MAX=247
 CONFIG_BT_CTLR_CONN_ISO_PDU_LEN_MAX=251
+CONFIG_BT_CTLR_CONN_ISO_LOW_LATENCY_POLICY=y
 
 # ISO Transmissions
-CONFIG_BT_CTLR_ISO_TX_BUFFERS=8
+CONFIG_BT_CTLR_ISOAL_SOURCES=4
+CONFIG_BT_CTLR_ISO_TX_BUFFERS=18
 CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
-CONFIG_BT_CTLR_ISOAL_SOURCES=2
 
 # ISO Receptions
-CONFIG_BT_CTLR_ISO_RX_BUFFERS=8
 CONFIG_BT_CTLR_ISOAL_SINKS=2
+CONFIG_BT_CTLR_ISO_RX_BUFFERS=8
 
 # Tx Power Dynamic Control
 CONFIG_BT_CTLR_TX_PWR_DYNAMIC_CONTROL=y
+
+# Ignore HCI ISO data Tx sequence numbers
+# CONFIG_BT_CTLR_ISOAL_PSN_IGNORE=y
diff --git a/samples/bluetooth/hci_uart_3wire/overlay-all-bt_ll_sw_split.conf b/samples/bluetooth/hci_uart_3wire/overlay-all-bt_ll_sw_split.conf
index df974a21ff4..8a0f9d3364a 100644
--- a/samples/bluetooth/hci_uart_3wire/overlay-all-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_uart_3wire/overlay-all-bt_ll_sw_split.conf
@@ -10,8 +10,8 @@ CONFIG_BT_EXT_ADV=y
 CONFIG_BT_PER_ADV=y
 CONFIG_BT_PER_ADV_SYNC=y
 CONFIG_BT_PER_ADV_SYNC_MAX=2
-CONFIG_BT_PERIPHERAL=y
 CONFIG_BT_CENTRAL=y
+CONFIG_BT_PERIPHERAL=y
 
 # Broadcast and Connected ISO
 CONFIG_BT_ISO_BROADCASTER=y
@@ -20,7 +20,11 @@ CONFIG_BT_ISO_CENTRAL=y
 CONFIG_BT_ISO_PERIPHERAL=y
 
 # ISO Streams
-CONFIG_BT_ISO_MAX_CHAN=2
+CONFIG_BT_ISO_TX_MTU=310
+CONFIG_BT_ISO_RX_MTU=310
+CONFIG_BT_ISO_MAX_CHAN=4
+CONFIG_BT_ISO_TX_BUF_COUNT=1
+CONFIG_BT_ISO_RX_BUF_COUNT=1
 
 # Controller
 CONFIG_BT_LL_SW_SPLIT=y
@@ -48,9 +52,16 @@ CONFIG_BT_CTLR_ADV_DATA_BUF_MAX=6
 
 # Increase the below to receive interleaved advertising chains
 CONFIG_BT_CTLR_SCAN_AUX_SET=1
+# CONFIG_BT_CTLR_SCAN_AUX_USE_CHAINS=y
+# CONFIG_BT_CTLR_SCAN_AUX_CHAIN_COUNT=1
 
 CONFIG_BT_CTLR_ADV_RESERVE_MAX=n
+CONFIG_BT_CTLR_ADV_ISO_RESERVE_MAX=y
+CONFIG_BT_CTLR_SYNC_ISO_RESERVE_MAX=n
 CONFIG_BT_CTLR_CENTRAL_RESERVE_MAX=n
+CONFIG_BT_CTLR_PERIPHERAL_RESERVE_MAX=n
+CONFIG_BT_CTLR_PERIPHERAL_ISO_RESERVE_MAX=n
+CONFIG_BT_CTLR_EVENT_OVERHEAD_RESERVE_MAX=y
 CONFIG_BT_CTLR_SLOT_RESERVATION_UPDATE=n
 CONFIG_BT_CTLR_SCAN_UNRESERVED=y
 CONFIG_BT_TICKER_NEXT_SLOT_GET_MATCH=y
@@ -82,31 +93,42 @@ CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_ADV_PERIODIC=y
 CONFIG_BT_CTLR_SYNC_TRANSFER_SENDER=y
 CONFIG_BT_CTLR_ADV_ISO=y
-CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
+CONFIG_BT_CTLR_ADV_ISO_SET=2
+CONFIG_BT_CTLR_ADV_ISO_STREAM_COUNT=4
 CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
+CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
 
 # ISO Receive Controller
 CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_SYNC_PERIODIC=y
 CONFIG_BT_CTLR_SYNC_TRANSFER_RECEIVER=y
 CONFIG_BT_CTLR_SYNC_ISO=y
-CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
+CONFIG_BT_CTLR_SCAN_SYNC_ISO_SET=1
+CONFIG_BT_CTLR_SYNC_ISO_STREAM_COUNT=2
 CONFIG_BT_CTLR_SYNC_ISO_STREAM_MAX=2
+CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
 
 # ISO Connection Oriented
 CONFIG_BT_CTLR_CENTRAL_ISO=y
 CONFIG_BT_CTLR_PERIPHERAL_ISO=y
+CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
+CONFIG_BT_CTLR_CONN_ISO_GROUPS=1
+CONFIG_BT_CTLR_CONN_ISO_STREAMS_PER_GROUP=2
 CONFIG_BT_CTLR_CONN_ISO_SDU_LEN_MAX=247
 CONFIG_BT_CTLR_CONN_ISO_PDU_LEN_MAX=251
+CONFIG_BT_CTLR_CONN_ISO_LOW_LATENCY_POLICY=y
 
 # ISO Transmissions
-CONFIG_BT_CTLR_ISO_TX_BUFFERS=8
+CONFIG_BT_CTLR_ISOAL_SOURCES=4
+CONFIG_BT_CTLR_ISO_TX_BUFFERS=18
 CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
-CONFIG_BT_CTLR_ISOAL_SOURCES=2
 
 # ISO Receptions
-CONFIG_BT_CTLR_ISO_RX_BUFFERS=8
 CONFIG_BT_CTLR_ISOAL_SINKS=2
+CONFIG_BT_CTLR_ISO_RX_BUFFERS=8
 
 # Tx Power Dynamic Control
 CONFIG_BT_CTLR_TX_PWR_DYNAMIC_CONTROL=y
+
+# Ignore HCI ISO data Tx sequence numbers
+# CONFIG_BT_CTLR_ISOAL_PSN_IGNORE=y