diff --git a/boards/arm/nucleo_f103rb/arduino_r3_connector.dtsi b/boards/arm/nucleo_f103rb/arduino_r3_connector.dtsi
index b866a53b6ce..3e887efebe2 100644
--- a/boards/arm/nucleo_f103rb/arduino_r3_connector.dtsi
+++ b/boards/arm/nucleo_f103rb/arduino_r3_connector.dtsi
@@ -36,3 +36,4 @@
 };
 
 arduino_spi: &spi1 {};
+arduino_i2c: &i2c1 {};
diff --git a/boards/arm/nucleo_f103rb/doc/index.rst b/boards/arm/nucleo_f103rb/doc/index.rst
index 2cdf51ca08d..350b39854ac 100644
--- a/boards/arm/nucleo_f103rb/doc/index.rst
+++ b/boards/arm/nucleo_f103rb/doc/index.rst
@@ -124,6 +124,7 @@ Default Zephyr Peripheral Mapping:
 - UART_2 TX/RX : PA2/PA3 (ST-Link Virtual COM Port)
 - SPI1 NSS/SCK/MISO/MOSI : PA4/PA5/PA6/PA7 (Arduino SPI)
 - SPI2 SCK/MISO/MOSI : PB12/PB13/PB14/PB15
+- I2C1 SDA/SCL: PB9/PB8 (Arduino I2C)
 - PWM1_CH1: PA8
 - USER_PB : PC13
 - LD1 : PA5
diff --git a/boards/arm/nucleo_f103rb/nucleo_f103rb.dts b/boards/arm/nucleo_f103rb/nucleo_f103rb.dts
index f6068ec2fce..9a734903643 100644
--- a/boards/arm/nucleo_f103rb/nucleo_f103rb.dts
+++ b/boards/arm/nucleo_f103rb/nucleo_f103rb.dts
@@ -58,6 +58,12 @@
 	current-speed = <115200>;
 };
 
+&i2c1 {
+	pinctrl-0 = <&i2c1_scl_pb8 &i2c1_sda_pb9>;
+	status = "okay";
+	clock-frequency = <I2C_BITRATE_FAST>;
+};
+
 &spi1 {
 	pinctrl-0 = <&spi1_nss_master_pa4 &spi1_sck_master_pa5
 		     &spi1_miso_master_pa6 &spi1_mosi_master_pa7>;
diff --git a/boards/arm/nucleo_f103rb/nucleo_f103rb.yaml b/boards/arm/nucleo_f103rb/nucleo_f103rb.yaml
index 790b7c9cec0..19ba971ea95 100644
--- a/boards/arm/nucleo_f103rb/nucleo_f103rb.yaml
+++ b/boards/arm/nucleo_f103rb/nucleo_f103rb.yaml
@@ -11,6 +11,7 @@ flash: 128
 supported:
   - arduino_gpio
   - arduino_spi
+  - arduino_i2c
   - gpio
   - spi
   - pwm