diff --git a/samples/boards/nordic/system_off/boards/nrf54l20pdk_nrf54l20_cpuapp.overlay b/samples/boards/nordic/system_off/boards/nrf54l20pdk_nrf54l20_cpuapp.overlay
deleted file mode 100644
index 3a79636a483..00000000000
--- a/samples/boards/nordic/system_off/boards/nrf54l20pdk_nrf54l20_cpuapp.overlay
+++ /dev/null
@@ -1,25 +0,0 @@
-/ {
-	cpuapp_sram@2007ec00 {
-		compatible = "zephyr,memory-region", "mmio-sram";
-		reg = <0x2007ec00 DT_SIZE_K(4)>;
-		zephyr,memory-region = "RetainedMem";
-		status = "okay";
-
-		retainedmem0: retainedmem {
-			compatible = "zephyr,retained-ram";
-			status = "okay";
-		};
-	};
-
-	aliases {
-		retainedmemdevice = &retainedmem0;
-	};
-};
-
-&cpuapp_sram {
-	/* Shrink SRAM size to avoid overlap with retained memory region:
-	 * 511 - 4 = 507KB = 0x7ec00
-	 */
-	reg = <0x20000000 DT_SIZE_K(507)>;
-	ranges = <0x0 0x20000000 0x7ec00>;
-};
diff --git a/samples/boards/nordic/system_off/sample.yaml b/samples/boards/nordic/system_off/sample.yaml
index 9b0e39a6cea..b11f3439e7f 100644
--- a/samples/boards/nordic/system_off/sample.yaml
+++ b/samples/boards/nordic/system_off/sample.yaml
@@ -14,7 +14,6 @@ tests:
       - nrf54l15dk/nrf54l05/cpuapp
       - nrf54l15dk/nrf54l10/cpuapp
       - nrf54l15dk/nrf54l15/cpuapp
-      - nrf54l20pdk/nrf54l20/cpuapp
       - nrf54lm20dk/nrf54lm20a/cpuapp
     harness: console
     harness_config:
@@ -34,7 +33,6 @@ tests:
       - nrf54l15dk/nrf54l05/cpuapp
       - nrf54l15dk/nrf54l10/cpuapp
       - nrf54l15dk/nrf54l15/cpuapp
-      - nrf54l20pdk/nrf54l20/cpuapp
       - nrf54lm20dk/nrf54lm20a/cpuapp
     extra_configs:
       - CONFIG_APP_USE_RETAINED_MEM=y
@@ -54,7 +52,6 @@ tests:
       - nrf54l15dk/nrf54l05/cpuapp
       - nrf54l15dk/nrf54l10/cpuapp
       - nrf54l15dk/nrf54l15/cpuapp
-      - nrf54l20pdk/nrf54l20/cpuapp
       - nrf54lm20dk/nrf54lm20a/cpuapp
     extra_configs:
       - CONFIG_GRTC_WAKEUP_ENABLE=y
@@ -79,7 +76,6 @@ tests:
       - nrf54l15dk/nrf54l05/cpuapp
       - nrf54l15dk/nrf54l10/cpuapp
       - nrf54l15dk/nrf54l15/cpuapp
-      - nrf54l20pdk/nrf54l20/cpuapp
       - nrf54lm20dk/nrf54lm20a/cpuapp
     extra_configs:
       - CONFIG_APP_USE_RETAINED_MEM=y