diff --git a/tests/drivers/i2c/i2c_api/boards/ek_ra8p1_r7ka8p1kflcac_cm85.conf b/tests/drivers/i2c/i2c_api/boards/ek_ra8p1_r7ka8p1kflcac_cm85.conf
new file mode 100644
index 00000000000..3b626dd7fad
--- /dev/null
+++ b/tests/drivers/i2c/i2c_api/boards/ek_ra8p1_r7ka8p1kflcac_cm85.conf
@@ -0,0 +1,4 @@
+# Copyright (c) 2025 Renesas Electronics Corporation
+# SPDX-License-Identifier: Apache-2.0
+
+CONFIG_SENSOR_GY271_QMC=y
diff --git a/tests/drivers/i2c/i2c_api/boards/ek_ra8p1_r7ka8p1kflcac_cm85.overlay b/tests/drivers/i2c/i2c_api/boards/ek_ra8p1_r7ka8p1kflcac_cm85.overlay
new file mode 100644
index 00000000000..7ce91203636
--- /dev/null
+++ b/tests/drivers/i2c/i2c_api/boards/ek_ra8p1_r7ka8p1kflcac_cm85.overlay
@@ -0,0 +1,16 @@
+/*
+ * Copyright (c) 2025 Renesas Electronics Corporation
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/ {
+	aliases {
+		i2c-0 = &iic1;
+		gy271 = &iic1;
+	};
+};
+
+&iic1 {
+	status = "okay";
+};
diff --git a/tests/drivers/i2c/i2c_api/boards/ek_ra8p1_r7ka8p1kflcac_cm85_sci_b_i2c.conf b/tests/drivers/i2c/i2c_api/boards/ek_ra8p1_r7ka8p1kflcac_cm85_sci_b_i2c.conf
new file mode 100644
index 00000000000..839f2b55341
--- /dev/null
+++ b/tests/drivers/i2c/i2c_api/boards/ek_ra8p1_r7ka8p1kflcac_cm85_sci_b_i2c.conf
@@ -0,0 +1,6 @@
+# Copyright (c) 2025 Renesas Electronics Corporation
+# SPDX-License-Identifier: Apache-2.0
+
+CONFIG_I2C=y
+CONFIG_ZTEST=y
+CONFIG_SENSOR_GY271_QMC=y
diff --git a/tests/drivers/i2c/i2c_api/boards/ek_ra8p1_r7ka8p1kflcac_cm85_sci_b_i2c.overlay b/tests/drivers/i2c/i2c_api/boards/ek_ra8p1_r7ka8p1kflcac_cm85_sci_b_i2c.overlay
new file mode 100644
index 00000000000..7aa8d7f0b0a
--- /dev/null
+++ b/tests/drivers/i2c/i2c_api/boards/ek_ra8p1_r7ka8p1kflcac_cm85_sci_b_i2c.overlay
@@ -0,0 +1,19 @@
+/*
+ * Copyright (c) 2025 Renesas Electronics Corporation
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/ {
+	aliases {
+		i2c-0 = &i2c1;
+		gy271 = &i2c1;
+	};
+};
+
+&sci1 {
+	status = "okay";
+
+	i2c1: i2c {
+		status = "okay";
+	};
+};
diff --git a/tests/drivers/i2c/i2c_api/testcase.yaml b/tests/drivers/i2c/i2c_api/testcase.yaml
index 3caf6281dea..f09762c135c 100644
--- a/tests/drivers/i2c/i2c_api/testcase.yaml
+++ b/tests/drivers/i2c/i2c_api/testcase.yaml
@@ -18,6 +18,17 @@ tests:
     extra_args:
       - DTC_OVERLAY_FILE="./boards/${BOARD}_sci_b_i2c.overlay"
       - CONF_FILE="./prj.conf ./boards/${BOARD}_sci_b_i2c.conf"
+  drivers.i2c.renesas_sci_b_i2c_ra8x2.api:
+    depends_on: i2c
+    tags:
+      - drivers
+      - i2c
+    filter: dt_alias_exists("gy271") and CONFIG_I2C_RENESAS_RA_SCI_B
+    platform_allow:
+      - ek_ra8p1/r7ka8p1kflcac/cm85
+    extra_args:
+      - DTC_OVERLAY_FILE="./boards/${BOARD}${NORMALIZED_BOARD_QUALIFIERS}_sci_b_i2c.overlay"
+      - CONF_FILE="./prj.conf ./boards/${BOARD}${NORMALIZED_BOARD_QUALIFIERS}_sci_b_i2c.conf"
   drivers.i2c.stm32.interrupt_disabled:
     depends_on:
       - i2c