diff --git a/tests/drivers/uart/uart_async_api/boards/nucleo_u385rg_q.overlay b/tests/drivers/uart/uart_async_api/boards/nucleo_u385rg_q.overlay
new file mode 100644
index 00000000000..87bd131e1ca
--- /dev/null
+++ b/tests/drivers/uart/uart_async_api/boards/nucleo_u385rg_q.overlay
@@ -0,0 +1,15 @@
+/*
+ * Copyright (c) 2025 STMicroelectronics
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+dut: &lpuart1 {
+	dmas = <&gpdma1 0 35 STM32_DMA_PERIPH_TX
+		&gpdma1 1 34 STM32_DMA_PERIPH_RX>;
+	dma-names = "tx", "rx";
+};
+
+&gpdma1 {
+	status = "okay";
+};