diff --git a/samples/drivers/uart/native_tty/boards/native_posix.overlay b/samples/drivers/uart/native_tty/boards/native_posix.overlay
deleted file mode 100644
index 6a3daca3241..00000000000
--- a/samples/drivers/uart/native_tty/boards/native_posix.overlay
+++ /dev/null
@@ -1 +0,0 @@
-#include "native_sim.overlay"